Overview

  • Product nameAnti-Dppa2 antibody
    See all Dppa2 primary antibodies
  • Description
    Rabbit polyclonal to Dppa2
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Dppa2.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: IOUD2; F9; Mouse EG; E14Tg2a.

Properties

Applications

Our Abpromise guarantee covers the use of ab91318 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 34 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • RelevanceDppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • cancer/testis antigen 100 antibody
    • CT100 antibody
    • Developmental Pluripotency Associated 2 antibody
    • developmental pluripotency-associated gene 2 antibody
    • developmental pluripotency-associated protein 2 antibody
    • ECAT15-2 antibody
    • embryonic stem cell (ESC) associated transcript 15-2 antibody
    • PESCRG1 antibody
    • Pluripotent embryonic stem cell related protein 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 protein antibody
    see all

Anti-Dppa2 antibody images

  • All lanes : Anti-Dppa2 antibody (ab91318) at 1 µg/ml

    Lane 1 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate
    Lane 4 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 34 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • ICC/IF image of ab91318 stained mouse embryonic stem cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab91318, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab91318, DyLight® 488 goat anti-rabbit IgX (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Dppa2 antibody (ab91318)

This product has been referenced in:
  • Tung PY  et al. Identification of DPPA4 and DPPA2 as a novel family of pluripotency-related oncogenes. Stem Cells N/A:N/A (2013). WB, IP, ChIP . Read more (PubMed: 23963736) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"