Anti-Dppa4 antibody (ab154642)
Key features and details
- Rabbit polyclonal to Dppa4
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dppa4 antibody
See all Dppa4 primary antibodies -
Description
Rabbit polyclonal to Dppa4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to a region within amino acids 30-304 of Human Dppa4 (UniProt ID:Q7L190).
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Positive control
- NT2D1 and Human ESC whole cell lysate. Human ESC cells. H661 xenograft.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 34 kDa.
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 34 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
May be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 55211 Human
- Omim: 614125 Human
- SwissProt: Q7L190 Human
- Unigene: 317659 Human
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Alternative names
- 2410091M23Rik antibody
- Developmental pluripotency associated 4 antibody
- Developmental pluripotency associated protein 4 antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded H661 (human cell line) xenograft tissue labelling Dppa4 with ab154642 at a 1/100 dilution.
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Anti-Dppa4 antibody (ab154642) at 1/500 dilution + NT2D1 whole cell lysate at 30 µg/ml
Predicted band size: 34 kDa
12% SDS PAGE -
Anti-Dppa4 antibody (ab154642) at 1/500 dilution + Human ESC whole cell lysate at 20 µg
Predicted band size: 34 kDa
12% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed Human ESC labeling Dppa4 with ab154642 at a 1/400 dilution.
The lower image has been stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154642 has been referenced in 2 publications.
- Nakai R et al. Derivation of induced pluripotent stem cells in Japanese macaque (Macaca fuscata). Sci Rep 8:12187 (2018). PubMed: 30111816
- Yu-Ching Lin Z et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Common marmoset . PubMed: 25624265