Overview

  • Product name
  • Description
    Rabbit polyclonal to Dppa4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human, Common marmoset
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 30-304 of Human Dppa4 (UniProt ID:Q7L190).

  • Positive control
    • NT2D1 and Human ESC whole cell lysate. Human ESC cells. H661 xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab154642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 34 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively Tris-EDTA buffer (pH8.0) may be used.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    May be involved in the maintenance of active epigenetic status of target genes (By similarity). May inhibit differentiation of embryonic cells into a primitive ectoderm lineage.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410091M23Rik antibody
    • Developmental pluripotency associated 4 antibody
    • Developmental pluripotency associated protein 4 antibody
    • Developmental pluripotency-associated protein 4 antibody
    • Dppa 4 antibody
    • DPPA4 antibody
    • DPPA4_HUMAN antibody
    • FLJ10713 antibody
    see all

Images

  • Anti-Dppa4 antibody (ab154642) at 1/500 dilution + NT2D1 whole cell lysate at 30 µg/ml

    Predicted band size: 34 kDa



    12% SDS PAGE
  • Anti-Dppa4 antibody (ab154642) at 1/500 dilution + Human ESC whole cell lysate at 20 µg

    Predicted band size: 34 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed Human ESC labeling Dppa4 with ab154642 at a 1/400 dilution.
    The lower image has been stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded H661 xenograft tissue labelling Dppa4 with ab154642 at a 1/100 dilution.

References

This product has been referenced in:
  • Yu-Ching Lin Z  et al. Gene expression ontogeny of spermatogenesis in the marmoset uncovers primate characteristics during testicular development. Dev Biol N/A:N/A (2015). IHC-P ; Common marmoset . Read more (PubMed: 25624265) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample
Marmoset (common) Tissue sections (adult testis)
Specification
adult testis
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. 煜欽 林

Verified customer

Submitted Jan 20 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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