The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use a concentration of 1 - 4 µg/ml. using PFA/Triton X-100 Fixation/Permeabilization.
As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. May also play an indirect or direct role in endosomal transport.
Immunocytochemistry/ Immunofluorescence - Anti-DPY30 antibody (ab126352)This image is courtesy of an anonymous Abreview
ab126352 staining DPY30 in Mouse NIH/3T3 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Tween 20 in PBS and blocked with 3% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (2μg/ml in 3% BSA + 0.2% Tween 20 + PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (4μg/ml in blocking solution for 1 hour) was used as the secondary antibody.
Lu F et al. Regulation of DNA replication and chromosomal polyploidy by the MLL-WDR5-RBBP5 methyltransferases. Biol Open5:1449-1460 (2016).
Read more (PubMed: 27744293) »
Ross NL et al. Histone-targeted Polyplexes Avoid Endosomal Escape and Enter the Nucleus During Postmitotic Redistribution of ER Membranes. Mol Ther Nucleic Acids4:e226 (2015).
Read more (PubMed: 25668340) »