Anti-DPY30 antibody - N-terminal (ab187690)

Overview

  • Product name
    Anti-DPY30 antibody - N-terminal
    See all DPY30 primary antibodies
  • Description
    Rabbit polyclonal to DPY30 - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human DPY30 aa 1-50 (N terminal). The exact sequence is proprietary. NP_115963.1.
    Sequence:

    MEPEQMLEGQTQVAENPHSEYGLTDNVERIVENEKINAEKSSKQKVDLQS


    Database link: Q9C005

  • Positive control
    • 293T whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab187690 was affinity purified using an epitope specific to DPY30 immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    Images

    • Detection of DPY30 by Western Blot of Immunoprecipitate.
      ab187690 at 1µg/ml labeling DPY30 in 293T whole cell lysate immunoprecipitated using ab187690 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 3 minutes.

      Please note: ab187690 has not performed satisfactorily when used for WB of DPY30 in crude preparations (e.g. whole cell lysate). It can be used for WB of enriched DPY30 (e.g. immunoprecipitated).

       

       

    References

    ab187690 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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