Overview

  • Product name
  • Description
    Mouse polyclonal to DR1
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Full length Human DR1 (NP_001929.1).

  • Positive control
    • WB: PC12, Raw 264.7, Jurkat, NIH/3T3 and HeLa nuclear cell lysates; DR1 transfected 293T cell lysate; ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab88597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
  • Sequence similarities
    Belongs to the NC2 beta/DR1 family.
    Contains 1 histone-fold domain.
  • Post-translational
    modifications
    Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Down regulator of transcription 1 antibody
    • Down regulator of transcription 1 TBP binding antibody
    • Down-regulator of transcription 1 antibody
    • down-regulator of transcription 1, TBP-binding (negative cofactor 2) antibody
    • DR 1 antibody
    • Dr1 antibody
    • Dr1 protein antibody
    • NC 2 antibody
    • NC2 antibody
    • NC2 beta antibody
    • NC2-beta antibody
    • NC2B_HUMAN antibody
    • Negative co factor 2 antibody
    • Negative cofactor 2 antibody
    • Negative cofactor 2 beta antibody
    • Negative cofactor 2-beta antibody
    • Protein Dr1 antibody
    • TATA binding protein associated phosphoprotein antibody
    • TATA box/binding protein associated phosphoprotein DR1 antibody
    • TATA-binding protein-associated phosphoprotein antibody
    see all

Images

  • Anti-DR1 antibody (ab88597) at 1 µg/ml + PC12 cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Additional bands at : 26 kDa. We are unsure as to the identity of these extra bands.
  • Anti-DR1 antibody (ab88597) at 1 µg/ml + HeLa nuclear cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Anti-DR1 antibody (ab88597) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • Anti-DR1 antibody (ab88597) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
  • Anti-DR1 antibody (ab88597) at 1 µg/ml + NIH3T3 cell lysate at 50 µg

    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • All lanes : Anti-DR1 antibody (ab88597) at 1 µg/ml

    Lane 1 : DR1 transfected 293T lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ab88597 at 10µg/ml staining DR1 in HeLa cells by Immunofluorescence.

References

This product has been referenced in:
  • Hsu SF  et al. A host susceptibility gene, DR1, facilitates influenza A virus replication by suppressing host innate immunity and enhancing viral RNA replication. J Virol 89:3671-82 (2015). Read more (PubMed: 25589657) »
  • Sun Z  et al. Functional divergence of the rapidly evolving miR-513 subfamily in primates. BMC Evol Biol 13:255 (2013). WB ; Human . Read more (PubMed: 24252134) »

See all 2 Publications for this product

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