Anti-DR1 antibody (ab88597)
Key features and details
- Mouse polyclonal to DR1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DR1 antibody
See all DR1 primary antibodies -
Description
Mouse polyclonal to DR1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant full length protein within Human DR1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- WB: PC12, Raw 264.7, Jurkat, NIH/3T3 and HeLa nuclear cell lysates; DR1 transfected 293T cell lysate; ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. -
Sequence similarities
Belongs to the NC2 beta/DR1 family.
Contains 1 histone-fold domain. -
Post-translational
modificationsPhosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1810 Human
- Entrez Gene: 13486 Mouse
- Entrez Gene: 289881 Rat
- Omim: 601482 Human
- SwissProt: Q01658 Human
- SwissProt: Q91WV0 Mouse
- SwissProt: Q5XI68 Rat
- Unigene: 348418 Human
see all -
Alternative names
- Down regulator of transcription 1 antibody
- Down regulator of transcription 1 TBP binding antibody
- Down-regulator of transcription 1 antibody
see all
Images
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Anti-DR1 antibody (ab88597) at 1 µg/ml + PC12 cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 26 kDa. We are unsure as to the identity of these extra bands. -
Anti-DR1 antibody (ab88597) at 1 µg/ml + HeLa nuclear cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Anti-DR1 antibody (ab88597) at 1 µg/ml + Raw 264.7 cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Anti-DR1 antibody (ab88597) at 1 µg/ml + Jurkat cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands. -
Anti-DR1 antibody (ab88597) at 1 µg/ml + NIH3T3 cell lysate at 50 µg
Predicted band size: 20 kDa
Observed band size: 20 kDa -
All lanes : Anti-DR1 antibody (ab88597) at 1 µg/ml
Lane 1 : DR1 transfected 293T lysate
Lane 2 : Non-transfected lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 20 kDa
Observed band size: 20 kDa -
ab88597 at 10µg/ml staining DR1 in HeLa cells by Immunofluorescence.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab88597 has been referenced in 3 publications.
- Wang T et al. Antiepileptic effect of uridine may be caused by regulating dopamine release and receptor expression in corpus striatum. Brain Res 1688:47-53 (2018). PubMed: 29555238
- Hsu SF et al. A host susceptibility gene, DR1, facilitates influenza A virus replication by suppressing host innate immunity and enhancing viral RNA replication. J Virol 89:3671-82 (2015). PubMed: 25589657
- Sun Z et al. Functional divergence of the rapidly evolving miR-513 subfamily in primates. BMC Evol Biol 13:255 (2013). WB ; Human . PubMed: 24252134