Recombinant Anti-DR1 antibody [EPR13122] (ab180164)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13122] to DR1
- Suitable for: IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DR1 antibody [EPR13122]
See all DR1 primary antibodies -
Description
Rabbit monoclonal [EPR13122] to DR1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Recombinant fragment within Human DR1. The exact sequence is proprietary.
Database link: Q01658 -
Positive control
- Human ovarian carcinoma and prostate tissues, HeLa, 293T, Jurkat and Human testis lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13122 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
1/10 - 1/100.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
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Notes |
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IP
1/10 - 1/100. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/1000 - 1/10000. Predicted molecular weight: 19 kDa. |
Target
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Function
The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4. -
Sequence similarities
Belongs to the NC2 beta/DR1 family.
Contains 1 histone-fold domain. -
Post-translational
modificationsPhosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1810 Human
- Entrez Gene: 13486 Mouse
- Entrez Gene: 289881 Rat
- Omim: 601482 Human
- SwissProt: Q01658 Human
- SwissProt: Q91WV0 Mouse
- SwissProt: Q5XI68 Rat
- Unigene: 348418 Human
see all -
Alternative names
- Down regulator of transcription 1 antibody
- Down regulator of transcription 1 TBP binding antibody
- Down-regulator of transcription 1 antibody
see all
Images
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All lanes : Anti-DR1 antibody [EPR13122] (ab180164) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : Human testis tissue lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 19 kDa -
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling DR1 using ab180164 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling DR1 using ab180164 at 1/250.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) pulling down DR1 using ab180164 at 1/10 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab180164 has not yet been referenced specifically in any publications.