Recombinant
RabMAb

Anti-DR1 antibody [EPR13122] (ab180164)

Overview

  • Product name
    Anti-DR1 antibody [EPR13122]
    See all DR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13122] to DR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt or ICC/IF
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human DR1. The exact sequence is proprietary.
    Database link: Q01658

  • Positive control
    • Human ovarian carcinoma and prostate tissues, HeLa, 293T, Jurkat and Human testis lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180164 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 19 kDa.
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.
  • Target

    • Function
      The association of the DR1/DRAP1 heterodimer with TBP results in a functional repression of both activated and basal transcription of class II genes. This interaction precludes the formation of a transcription-competent complex by inhibiting the association of TFIIA and/or TFIIB with TBP. Can bind to DNA on its own. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
    • Sequence similarities
      Belongs to the NC2 beta/DR1 family.
      Contains 1 histone-fold domain.
    • Post-translational
      modifications
      Phosphorylation regulates its interaction with TBP. Not phosphorylated when bound to DRAP1.
    • Cellular localization
      Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Down regulator of transcription 1 antibody
      • Down regulator of transcription 1 TBP binding antibody
      • Down-regulator of transcription 1 antibody
      • down-regulator of transcription 1, TBP-binding (negative cofactor 2) antibody
      • DR 1 antibody
      • Dr1 antibody
      • Dr1 protein antibody
      • NC 2 antibody
      • NC2 antibody
      • NC2 beta antibody
      • NC2-beta antibody
      • NC2B_HUMAN antibody
      • Negative co factor 2 antibody
      • Negative cofactor 2 antibody
      • Negative cofactor 2 beta antibody
      • Negative cofactor 2-beta antibody
      • Protein Dr1 antibody
      • TATA binding protein associated phosphoprotein antibody
      • TATA box/binding protein associated phosphoprotein DR1 antibody
      • TATA-binding protein-associated phosphoprotein antibody
      see all

    Images

    • All lanes : Anti-DR1 antibody [EPR13122] (ab180164) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : Human testis lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 19 kDa

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling DR1 using ab180164 at 1/250.

    • Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling DR1 using ab180164 at 1/250.

    • Western blot analysis on immunoprecipitation pellet from (1) 293T cell lysate or (2) 1X PBS (negative control) pulling down DR1 using ab180164 at 1/10 and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    References

    ab180164 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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