Overview

  • Product name
  • Description
    Rabbit polyclonal to DR3
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    APCTEPCGNSTCLVCPQDT

    , corresponding to extracellular amino acids 59-77 of Human DR3 (Peptide available as ab8444.)

  • Positive control
    • Jurkat total cell lysate A 59 kDa band should be detected.

Properties

Applications

Our Abpromise guarantee covers the use of ab8412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 59 kDa.Can be blocked with Human DR3 peptide (ab8444).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Receptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
  • Tissue specificity
    Abundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.
  • Sequence similarities
    Contains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo 3 antibody
    • Apo-3 antibody
    • Apo3 antibody
    • Apoptosis inducing receptor AIR antibody
    • Apoptosis inducing receptor antibody
    • Apoptosis mediating receptor antibody
    • Apoptosis mediating receptor DR 3 antibody
    • Apoptosis mediating receptor DR3 antibody
    • Apoptosis mediating receptor TRAMP antibody
    • Apoptosis-inducing receptor AIR antibody
    • Apoptosis-mediating receptor DR3 antibody
    • Apoptosis-mediating receptor TRAMP antibody
    • DDR 3 antibody
    • DDR3 antibody
    • Death domain receptor 3 antibody
    • Death domain receptor 3 soluble form antibody
    • Death receptor 3 antibody
    • Death receptor beta antibody
    • DR 3 antibody
    • DR3 antibody
    • LARD antibody
    • Lymphocyte associated receptor of death antibody
    • Lymphocyte-associated receptor of death antibody
    • Protein WSL antibody
    • Protein WSL-1 antibody
    • TNF receptor superfamily member 25 antibody
    • TNFR25 antibody
    • TNFRSF 12 antibody
    • TNFRSF 25 antibody
    • TNFRSF12 antibody
    • TNFRSF12, formerly antibody
    • TNFRSF25 antibody
    • TNR25_HUMAN antibody
    • TR 3 antibody
    • TR3 antibody
    • TRAMP antibody
    • Translocating chain association membrane protein antibody
    • Tumor necrosis factor receptor superfamily member 12 antibody
    • Tumor necrosis factor receptor superfamily member 25 antibody
    • Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein) antibody
    • Tumor necrosis factor receptor superfamily, member 12, formerly antibody
    • WSL 1 antibody
    • WSL antibody
    • WSL LR antibody
    • WSL protein antibody
    • WSL1 antibody
    • WSL1 protein antibody
    • WSLLR antibody
    see all

Images

  • Anti-DR3 antibody (ab8412) at 1/1000 dilution + Jurkat total cell lysate

    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
  • ab8412 at 10µg/ml staining DR3 in Jurkat cells by ICC/IF 

References

ab8412 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Rat Tissue sections (Retina)
Specification
Retina
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Retina)
Specification
Retina
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (SHSY5Y)
Specification
SHSY5Y
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Cell (SHSY5Y)
Specification
SHSY5Y
Permeabilization
No
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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