Overview

  • Product nameAnti-DR3 antibody
    See all DR3 primary antibodies
  • Description
    Rabbit polyclonal to DR3
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    APCTEPCGNSTCLVCPQDT

    , corresponding to extracellular amino acids 59-77 of Human DR3 (Peptide available as ab8444.)

  • Positive control
    • Jurkat total cell lysate A 59 kDa band should be detected.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab8412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 59 kDa.Can be blocked with Human DR3 peptide (ab8444).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionReceptor for TNFSF12/APO3L/TWEAK. Interacts directly with the adapter TRADD. Mediates activation of NF-kappa-B and induces apoptosis. May play a role in regulating lymphocyte homeostasis.
  • Tissue specificityAbundantly expressed in thymocytes and lymphocytes. Detected in lymphocyte-rich tissues such as thymus, colon, intestine, and spleen. Also found in the prostate.
  • Sequence similaritiesContains 1 death domain.
    Contains 4 TNFR-Cys repeats.
  • Post-translational
    modifications
    Glycosylated.
  • Cellular localizationSecreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo 3 antibody
    • Apo-3 antibody
    • Apo3 antibody
    • Apoptosis inducing receptor AIR antibody
    • Apoptosis inducing receptor antibody
    • Apoptosis mediating receptor antibody
    • Apoptosis mediating receptor DR 3 antibody
    • Apoptosis mediating receptor DR3 antibody
    • Apoptosis mediating receptor TRAMP antibody
    • Apoptosis-inducing receptor AIR antibody
    • Apoptosis-mediating receptor DR3 antibody
    • Apoptosis-mediating receptor TRAMP antibody
    • DDR 3 antibody
    • DDR3 antibody
    • Death domain receptor 3 antibody
    • Death domain receptor 3 soluble form antibody
    • Death receptor 3 antibody
    • Death receptor beta antibody
    • DR 3 antibody
    • DR3 antibody
    • LARD antibody
    • Lymphocyte associated receptor of death antibody
    • Lymphocyte-associated receptor of death antibody
    • Protein WSL antibody
    • Protein WSL-1 antibody
    • TNF receptor superfamily member 25 antibody
    • TNFRSF 12 antibody
    • TNFRSF 25 antibody
    • TNFRSF12 antibody
    • TNFRSF25 antibody
    • TNR25_HUMAN antibody
    • TR 3 antibody
    • TR3 antibody
    • TRAMP antibody
    • Translocating chain association membrane protein antibody
    • Tumor necrosis factor receptor superfamily member 12 antibody
    • Tumor necrosis factor receptor superfamily member 25 antibody
    • Tumor necrosis factor receptor superfamily, member 12 (translocating chain association membrane protein) antibody
    • WSL 1 antibody
    • WSL antibody
    • WSL LR antibody
    • WSL protein antibody
    • WSL1 antibody
    • WSL1 protein antibody
    • WSLLR antibody
    see all

Anti-DR3 antibody images

  • Anti-DR3 antibody (ab8412) at 1/1000 dilution + Jurkat total cell lysate

    Observed band size : 59 kDa (why is the actual band size different from the predicted?)
  • ab8412 at 10µg/ml staining DR3 in Jurkat cells by ICC/IF 

References for Anti-DR3 antibody (ab8412)

ab8412 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Rat Tissue sections (Retina)
Specification Retina
Permeabilization No
Fixative Paraformaldehyde
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Submitted Apr 28 2014

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Retina)
Specification Retina
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative Paraformaldehyde
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Submitted Apr 28 2014

Application Immunocytochemistry
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (SHSY5Y)
Specification SHSY5Y
Permeabilization No
Fixative Paraformaldehyde
Username

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Submitted Apr 28 2014

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Sample Human Cell (SHSY5Y)
Specification SHSY5Y
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Apr 28 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"