Overview

  • Product nameAnti-DR4 antibody
    See all DR4 primary antibodies
  • Description
    Rabbit polyclonal to DR4
  • SpecificityDoes not cross react with DR5.
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CDSGKFIYLEDGTGSAVSLE

    , corresponding to amino acids 450/468 of Human DR4 (Peptide available as ab8446.)

  • Positive control
    • HeLa whole cell lysate A 57 kDa band should be detected.

Properties

Applications

Our Abpromise guarantee covers the use of ab8414 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/500 - 1/1000. Detects a band of approximately 57 kDa.Can be blocked with Human DR4 peptide (ab8446).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionReceptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
  • Tissue specificityWidely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo2 antibody
    • CD261 antibody
    • Cytotoxic TRAIL receptor antibody
    • Death receptor 4 antibody
    • DR4 antibody
    • NF related apoptosis-inducing ligand receptor 1 antibody
    • TNF receptor superfamily member 10a antibody
    • TNF-related apoptosis-inducing ligand receptor 1 antibody
    • TNFRSF10A antibody
    • TR10A_HUMAN antibody
    • TRAIL receptor 1 antibody
    • TRAIL-R1 antibody
    • TRAILR 1 antibody
    • TRAILR1 antibody
    • Tumor necrosis factor receptor superfamily member 10A antibody
    • Tumor necrosis factor receptor superfamily member 10a variant 2 antibody
    • Tumor necrosis factor receptor superfamily, member 10a antibody
    see all

Anti-DR4 antibody images

  • Anti-DR4 antibody (ab8414) at 1/500 dilution + HeLa total cell lysate

    Observed band size : 57 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry of DR4 in human spleen tissue with DR4 antibody at 5 µg/ml.

  • ab8414 at 10µg/ml staining DR4 in Jurkat cells by ICC/IF

References for Anti-DR4 antibody (ab8414)

This product has been referenced in:
  • Akram KM  et al. Alveolar epithelial cells in idiopathic pulmonary fibrosis display upregulation of TRAIL, DR4 and DR5 expression with simultaneous preferential over-expression of pro-apoptotic marker p53. Int J Clin Exp Pathol 7:552-64 (2014). IHC-P ; Human . Read more (PubMed: 24551275) »
  • Fröhlich LF  et al. Epigenetic silencing of apoptosis-inducing gene expression can be efficiently overcome by combined SAHA and TRAIL treatment in uterine sarcoma cells. PLoS One 9:e91558 (2014). WB ; Human . Read more (PubMed: 24618889) »

See all 9 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate pH6
Permeabilization Yes - TBST
Blocking step 2%BSA/2% NGS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Username

Miss. Sandra Peternel

Verified customer

Submitted Jan 27 2010

Thank you for your enquiry. Ab8414 has been successfully tested for application in Western blotting and ab8416 has been successfully tested for application in Western blotting and IHC-P (see customer review). We currently do not have any information ab...

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All the information that we have received from the customer is located in the reviews section of the online datasheet. He did not mention what type of samples he used, but I assume that they were from either human, rat or mouse. You can click on the cu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"