CDSGKFIYLEDGTGSAVSLE, corresponding to amino acids 450/468 of Human DR4 (Peptide available as ab8446.)
Our Abpromise guarantee covers the use of ab8414 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 57 kDa.Can be blocked with Human DR4 peptide (ab8446).|
|ICC/IF||Use a concentration of 10 µg/ml.|
Immunohistochemistry of DR4 in human spleen tissue with DR4 antibody at 5 µg/ml.
Immunofluorescent analysis of human spleen tissue labeling DR4 with ab8414 at 20 ug/ml.
ab8414 at 10µg/ml staining DR4 in Jurkat cells by ICC.