Overview

  • Product nameAnti-DR4 antibody [DR-4-02] (FITC)
    See all DR4 primary antibodies
  • Description
    Mouse monoclonal [DR-4-02] to DR4 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityThe antibody recognizes TRAIL R1 (DR4), a human death receptor 4 (468 amino acids) expressed in most human tissues (spleen, peripheral blood leucocytes, thymus) and in a variety of tumour derived cell lines.
  • Tested applicationsSuitable for: IP, Functional Studies, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein (Human) containing the extracellular part of DR4 and the constant part of the heavy chain of human IgG1.

  • General notesDo not use after expiration date stamped on vial label. Short term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.


    Soluble antibody blocks DR4 receptor against TRAIL induced apoptosis. Plastic immobilized antibody induces apoptosis in sensitive cells.

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% high grade protease free BSA, PBS
  • Concentration information loading...
  • PuritySize exclusion
  • Purification notesThe antibody was >95% pure prior to conjugation as determined from SDS-PAGE (by protein A affinity chromatography).
  • Primary antibody notesSoluble antibody blocks DR4 receptor against TRAIL induced apoptosis. Plastic immobilized antibody induces apoptosis in sensitive cells.
  • ClonalityMonoclonal
  • Clone numberDR-4-02
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab59047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Functional Studies Use a concentration of 2 - 3 µg/ml. in cultivation medium. Final concentration of TRAIL: 20-200 ng/ml. Note: It is recommended that the antibody is added 15 min before the addition of TRAIL.
Flow Cyt Use a concentration of 5 µg/ml. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • FunctionReceptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B.
  • Tissue specificityWidely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K562 erythroleukemia cells, MCF7 breast carcinoma cells and activated T-cells.
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apo2 antibody
    • CD261 antibody
    • Cytotoxic TRAIL receptor antibody
    • Death receptor 4 antibody
    • DR4 antibody
    • NF related apoptosis-inducing ligand receptor 1 antibody
    • TNF receptor superfamily member 10a antibody
    • TNF-related apoptosis-inducing ligand receptor 1 antibody
    • TNFRSF10A antibody
    • TR10A_HUMAN antibody
    • TRAIL receptor 1 antibody
    • TRAIL-R1 antibody
    • TRAILR 1 antibody
    • TRAILR1 antibody
    • Tumor necrosis factor receptor superfamily member 10A antibody
    • Tumor necrosis factor receptor superfamily member 10a variant 2 antibody
    • Tumor necrosis factor receptor superfamily, member 10a antibody
    see all

References for Anti-DR4 antibody [DR-4-02] (FITC) (ab59047)

This product has been referenced in:
  • Nesterenko I  et al. Evaluating the effect of therapeutic stem cells on TRAIL resistant and sensitive medulloblastomas. PLoS One 7:e49219 (2012). Flow Cyt ; Human . Read more (PubMed: 23145127) »
  • Fan S  et al. Scatter factor protects tumor cells against apoptosis caused by TRAIL. Anticancer Drugs 21:10-24 (2010). IP . Read more (PubMed: 19823077) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"