Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab16329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 58 kDa.

Target

  • FunctionReceptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
  • Tissue specificityWidely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
  • Involvement in diseaseSquamous cell carcinoma of the head and neck
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fas like protein antibody
    • Apoptosis inducing protein TRICK2A/2B antibody
    • Apoptosis inducing receptor TRAIL R2 antibody
    • CD262 antibody
    • CD262 antigen antibody
    • Cytotoxic TRAIL receptor 2 antibody
    • Death domain containing receptor for TRAIL/Apo 2L antibody
    • Death receptor 5 antibody
    • DR5 antibody
    • KILLER antibody
    • KILLER/DR5 antibody
    • OTTHUMP00000123492 antibody
    • OTTHUMP00000123493 antibody
    • p53 regulated DNA damage inducible cell death receptor(killer) antibody
    • TNF related apoptosis inducing ligand receptor 2 antibody
    • TNF-related apoptosis-inducing ligand receptor 2 antibody
    • TNFRSF10B antibody
    • TR10B_HUMAN antibody
    • TRAIL R2 antibody
    • TRAIL receptor 2 antibody
    • TRAIL-R2 antibody
    • TRAILR2 antibody
    • TRICK2 antibody
    • TRICK2A antibody
    • TRICK2B antibody
    • TRICKB antibody
    • Tumor necrosis factor receptor like protein ZTNFR9 antibody
    • Tumor necrosis factor receptor superfamily member 10B antibody
    • Tumor necrosis factor receptor superfamily, member 10b antibody
    • ZTNFR9 antibody
    see all

Anti-DR5 antibody images

  • All lanes : Anti-DR5 antibody (ab16329) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC12 cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    HRP conjugated anti-rabbit
    Developed using the ECL technique

    Predicted band size : 58 kDa
    Observed band size : 58 kDa

References for Anti-DR5 antibody (ab16329)

This product has been referenced in:
  • Catlin NR  et al. The Stage-specific Testicular Germ Cell Apoptotic Response to Low-dose Radiation and 2,5-hexanedione Combined Exposure. II: qRT-PCR Array Analysis Reveals Dose Dependent Adaptive Alterations in the Apoptotic Pathway. Toxicol Pathol N/A:N/A (2014). Rat . Read more (PubMed: 24670816) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Non-reduced Denaturing (12% gel)
Sample Rat Tissue lysate - whole (Testis)
Specification Testis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

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Verified customer

Submitted May 16 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"