• Product nameAnti-DR5 antibody
    See all DR5 primary antibodies
  • Description
    Rabbit polyclonal to DR5
  • SpecificityDoes not cross react with DR4.
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human DR5 aa 388-407. DR5 antibody was raised against a peptide corresponding to 20 amino acids near the carboxy terminus of human DR5 precursor. The immunogen is located within the last 50 amino acids of DR5.
    (Peptide available as ab8449)

  • Positive control
    • Whole cell lysate from HeLa cells



Our Abpromise guarantee covers the use of ab8416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/250 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa).Can be blocked with Human DR5 peptide (ab8449).
ICC/IF Use a concentration of 5 µg/ml.


  • FunctionReceptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
  • Tissue specificityWidely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
  • Involvement in diseaseSquamous cell carcinoma of the head and neck
  • Sequence similaritiesContains 1 death domain.
    Contains 3 TNFR-Cys repeats.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Fas like protein antibody
    • Apoptosis inducing protein TRICK2A/2B antibody
    • Apoptosis inducing receptor TRAIL R2 antibody
    • CD262 antibody
    • CD262 antigen antibody
    • Cytotoxic TRAIL receptor 2 antibody
    • Death domain containing receptor for TRAIL/Apo 2L antibody
    • Death receptor 5 antibody
    • DR5 antibody
    • KILLER antibody
    • KILLER/DR5 antibody
    • OTTHUMP00000123492 antibody
    • OTTHUMP00000123493 antibody
    • p53 regulated DNA damage inducible cell death receptor(killer) antibody
    • TNF related apoptosis inducing ligand receptor 2 antibody
    • TNF-related apoptosis-inducing ligand receptor 2 antibody
    • TNFRSF10B antibody
    • TR10B_HUMAN antibody
    • TRAIL R2 antibody
    • TRAIL receptor 2 antibody
    • TRAIL-R2 antibody
    • TRAILR2 antibody
    • TRICK2 antibody
    • TRICK2A antibody
    • TRICK2B antibody
    • TRICKB antibody
    • Tumor necrosis factor receptor like protein ZTNFR9 antibody
    • Tumor necrosis factor receptor superfamily member 10B antibody
    • Tumor necrosis factor receptor superfamily, member 10b antibody
    • ZTNFR9 antibody
    see all

Anti-DR5 antibody images

  • All lanes : Anti-DR5 antibody (ab8416) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : K562 whole cell lysate

    Predicted band size : 47.8 kDa
  • ab8416 at 5µg/ml staining DR5 in Hela cells by ICC/IF.

  • The image above is a xenograph of human cancer cells implanted into a nude mouse and allowed to grow for about three weeks. Notice only the human cancer cells expressed this antigen. This picture was kindly submitted as part of the reveiw by Dean Wilkie.

References for Anti-DR5 antibody (ab8416)

This product has been referenced in:
  • Akram KM  et al. Alveolar epithelial cells in idiopathic pulmonary fibrosis display upregulation of TRAIL, DR4 and DR5 expression with simultaneous preferential over-expression of pro-apoptotic marker p53. Int J Clin Exp Pathol 7:552-64 (2014). IHC-P ; Human . Read more (PubMed: 24551275) »
  • Fröhlich LF  et al. Epigenetic silencing of apoptosis-inducing gene expression can be efficiently overcome by combined SAHA and TRAIL treatment in uterine sarcoma cells. PLoS One 9:e91558 (2014). WB ; Human . Read more (PubMed: 24618889) »

See all 11 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (nasopharynx)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6,0
Permeabilization No
Specification nasopharynx
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 01 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Hela cells)
Permeabilization No
Specification Hela cells
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative Methanol

Abcam user community

Verified customer

Submitted Sep 23 2015

Application Western blot
Sample Human Tissue lysate - whole (skin biopsie)
Gel Running Conditions Reduced Non-Denaturing (Native) (5-16% gel)
Loading amount 15 µg
Treatment see below
Specification skin biopsie
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Sep 22 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (Mesothelioma)
Specification Mesothelioma
Treatment siRNA and Radiation
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C

Dr. Saurabh Dayal

Verified customer

Submitted Sep 02 2014

This antibody has been tested for mouse in Western Blot although the image is not available.
At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform ...

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Thank you for contacting us.

We normally issue the discount code prior to buying and testing the product. However, in this case, I am happy to make an exception. I have just issued a new code.

Thank you for your Abreview on this p...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblast)
Loading amount 30 µg
Specification Mouse embryonic fibroblast
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 07 2012

Thank you very much for your interest in ab8416

We do not currently have an image of this antibody in western blot or IHC Therefore, I can offer a discount off a future purchase if you buy ab8416 now, use it in western blot or IHC and submit...

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Thank you for your enquiry. Ab8414 has been successfully tested for application in Western blotting and ab8416 has been successfully tested for application in Western blotting and IHC-P (see customer review). We currently do not have any information ab...

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Thank you for your question regarding the MW of DR5. The predicted MW of the human precursor protein is 47.8kDa according to SwissProt and the antibody ab8416 recognised a band just above the 55kDa MW marker in HeLa and K562 whole cell lysates. I h...

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