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KWVNKTGRDASVHTLLDALE, corresponding to amino acids 388-407 of Human DR5 (Peptide available as ab8449.)
Our Abpromise guarantee covers the use of ab8416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|WB||1/250 - 1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 47.8 kDa).Can be blocked with Human DR5 peptide (ab8449).|
|ICC/IF||Use a concentration of 5 µg/ml.|
The image above is a xenograph of human cancer cells implanted into a nude mouse and allowed to grow for about three weeks. Notice only the human cancer cells expressed this antigen. This picture was kindly submitted as part of the reveiw by Dean Wilkie.
ab8416 at 5µg/ml staining DR5 in Hela cells by ICC/IF