The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 40, 48 kDa (predicted molecular weight: 47 kDa).
1/400. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF-kappa-B. Essential for ER stress-induced apoptosis.
Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
Involvement in disease
Squamous cell carcinoma of the head and neck
Contains 1 death domain. Contains 3 TNFR-Cys repeats.
Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling DR5 with ab181846 at 1/400 dilution (red) compared to a Rabbit monoclonal IgG Isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Western blot - Anti-DR5 [EPR1659(2)] antibody (ab181846)
All lanes : Anti-DR5 antibody [EPR1659(2)] (ab181846) at 1/50000 dilution
Lane 1 : HepG2 cell lysate Lane 2 : HeLa cell lysate Lane 3 : HT1080 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Western blot analysis of HeLa cell lysate immunoprecipitated using ab181846 at 1/30 dilution (Lane 1). Lane 2: Negative control. Anti-Rabbit IgG (HRP) secondary antibody, specific to the non-reduced form of IgG was used at 1/1500 dilution.