Overview

  • Product nameAnti-DRAK1 antibody
    See all DRAK1 primary antibodies
  • Description
    Rabbit polyclonal to DRAK1
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • Immunogen

    A region between residue 354 and 414 of Human DRAK1 (NP_004751.2)

  • Positive control
    • Whole cell lysate from HeLa cells and 293T cells

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab95184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 47 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionActs as a positive regulator of apoptosis. Also acts as a regulator of cellular reactive oxygen species.
  • Tissue specificityHighly expressed in placenta. Lower levels in heart, lung, skeletal muscle, kidney and pancreas.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP kinase-related apoptosis-inducing protein kinase 1 antibody
    • death-associated protein kinase-related 1 antibody
    • DRAK1 antibody
    • EC 2.7.11.1 antibody
    • Serine / threonine kinase 17a death associated protein kinase related 1 antibody
    • Serine/threonine kinase 17a antibody
    • Serine/threonine protein kinase 17a antibody
    • Serine/threonine-protein kinase 17A antibody
    • ST17A_HUMAN antibody
    • STK17 A antibody
    • STK17A antibody
    see all

Anti-DRAK1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling DRAK1 with ab95184 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-DRAK1 antibody (ab95184) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa at 50 µg
    Lane 2 : Whole cell lysate from HeLa at 15 µg
    Lane 3 : Whole cell lysate from HeLa at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 47 kDa


    Exposure time : 30 seconds
  • Detection of Human DRAK1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (1 mg for IP, 20% of IP loaded). Antibodies: ab95184 was used for WB at 0.4 ug/ml and used for IP at 3 ug/mg lysate (lane 2). DRAK1 was also immunoprecipitated by another rabbit DRAK1 antibody (lane 1) and control IgG (lane 3). Detection: Chemiluminescence with exposure times of 10 seconds.

References for Anti-DRAK1 antibody (ab95184)

ab95184 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"