Anti-DRAK2 antibody (ab8419)

Overview

  • Product nameAnti-DRAK2 antibody
    See all DRAK2 primary antibodies
  • Description
    Rabbit polyclonal to DRAK2
  • SpecificityNone to DAP or ZIP kinases. The approximately 70 kDa band visible on Western blots is probably non-related to DRAK2 although it is peptide blockable with ab8454.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    CSKRFRFDDSLPNPHE

    , corresponding to amino acids 351/365 of Human DRAK2. (Peptide available as ab8454.)

  • Positive control
    • Wholecell lysate from Jurkat cells An approximately 45 kDa band can be detected.
  • General notes


    Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPBS with 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesDRAK2 Antibody is Ion exchange chromatography purified.
  • Primary antibody notesApoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP,are mediators of apoptosis. Two novel serine/threonine kinases that induce apoptosis were recently identified and designated DRAK1 and DRAK2 (for DAP kinase-related apoptosis-inducing protein kinases) (1). DRAKs contain anN-terminal kinase domain and a C-terminal regulation domain. Overexpression of DRAK2 induces apoptosis. DRAKs have high sequence homology to DAP and ZIP kinases, and they represent a novel family of serine/threonine kinases, which mediates apoptosis through their catalytic activities. DRAK2 is located in nucleus and the messenger RNA was ubiquitously expressed in human tissues.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Light chain typeunknown
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab8419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 43 kDa).Can be blocked with Human DRAK2 peptide (ab8454).
ICC/IF Use a concentration of 10 µg/ml.

Target

  • FunctionActs as a positive regulator of apoptosis.
  • Tissue specificityHighly expressed in placenta, lung, pancreas. Lower levels in heart, brain, liver, skeletal muscle and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications
    Autophosphorylated.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DAP kinase related apoptosis inducing protein antibody
    • DAP kinase related apoptosis inducing protein kinase 2 antibody
    • DAP kinase-related apoptosis-inducing protein kinase 2 antibody
    • Death associated protein kinase related 2 antibody
    • DRAK 2 antibody
    • DRAK2 antibody
    • Serine/threonine kinase 17b antibody
    • Serine/threonine kinase 17b apoptosis inducing antibody
    • Serine/threonine protein kinase 17B antibody
    • Serine/threonine-protein kinase 17B antibody
    • ST17B_HUMAN antibody
    • STK 17B antibody
    • Stk17b antibody
    see all

Anti-DRAK2 antibody images

  • Lane 1 : Anti-DRAK2 antibody (ab8419) at 1 µg/ml (DRAK2 antibody)
    Lane 2 : Anti-DRAK2 antibody (ab8419) at 2 µg/ml (DRAK2 antibody)

    Lane 1 : Raji cell lysate
    Lane 2 : Raji cell lysate


    Predicted band size : 43 kDa
  • All lanes : Anti-DRAK2 antibody (ab8419) at 1/500 dilution

    Lane 1 : Jurkat whole cell lysate with absence of blocking peptide
    Lane 2 : Raji whole cell lysate with absence of blocking peptide
    Lane 3 : Jurkat whole cell lysate with presence of blocking peptide
    Lane 4 : Raji whole cell lysate with presence of blocking peptide


    Predicted band size : 43 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    We are unsure about the nature of the 70 kDa band. However, DRAK2 is autophosphorylated and it is possible that this band corresponds to the phosphorylated form. The fact that the detection of this band is blocked by DRAK2 peptide indicates that it probably is closely related to the DRAK2 protein.
  • ab8419 at 10µg/ml staining DRAK2 in Jurkat cells by ICC/IF

References for Anti-DRAK2 antibody (ab8419)

ab8419 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (B16F10 murine melanoma cell line)
Loading amount 40 µg
Specification B16F10 murine melanoma cell line
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%
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Abcam user community

Verified customer

Submitted Aug 04 2006

Cross-reaction for peptide antibodies is not uncommon, the band at 42 kDa is specific to DRAK2, but the 70 kDa band is likely to be against a different protein from our own internal testing.

Thank you for your enquiry. It is unnecessary to isolate nuclear proteins before running a western blot. You can detect DRAK2 by loading the Jurkat whole cell lysate directly onto the gel.

Thank you for your enquiry. The larger band is probably a cross-reaction to a non-related protein or a different member of the same family. We would suggest trying longer exposure of the membrane to x-ray film (30 minutes to overnight). It is ...

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