Anti-DRAM antibody [M3-P4B4] (ab64739)

Overview

  • Product nameAnti-DRAM antibody [M3-P4B4]
    See all DRAM primary antibodies
  • Description
    Mouse monoclonal [M3-P4B4] to DRAM
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from within residues 150 - 250 of Human DRAM.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab64739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 26 kDa.
  • Application notesIs unsuitable for ICC/IF.
  • Target

    • FunctionLysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.
    • Sequence similaritiesBelongs to the DRAM/TMEM150 family.
    • Cellular localizationLysosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Damage regulated autophagy modulator antibody
      • Damage-regulated autophagy modulator 1 antibody
      • Damage-regulated autophagy modulator antibody
      • DNA damage-regulated autophagy modulator protein 1 antibody
      • DRAM antibody
      • dram1 antibody
      • DRAM1_HUMAN antibody
      • FLJ11259 antibody
      see all

    Anti-DRAM antibody [M3-P4B4] images

    • Anti-DRAM antibody [M3-P4B4] (ab64739) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 26 kDa
      Observed band size : 26 kDa


      Exposure time : 20 minutes

    References for Anti-DRAM antibody [M3-P4B4] (ab64739)

    ab64739 has not yet been referenced specifically in any publications.

    Product Wall

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    I have checked the history of this antibody and can confirm that we haven't received specificity complaints for this product so I am sure protocol troubleshooting will help in getting a clean wes...

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    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (glioma stem cells)
    Specification glioma stem cells
    Fixative Paraformaldehyde
    Permeabilization Yes - triton-x 0.1%
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 26 2012

    Application Western blot
    Sample Mouse Tissue lysate - whole (HeLa Ms Stomach Ms Kidney Ms Liver)
    Loading amount 20 µg
    Specification HeLa Ms Stomach Ms Kidney Ms Liver
    Gel Running Conditions Reduced Denaturing
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"