Anti-DRAM antibody [M3-P4B4] (ab64739)

Overview

  • Product name
    Anti-DRAM antibody [M3-P4B4]
    See all DRAM primary antibodies
  • Description
    Mouse monoclonal [M3-P4B4] to DRAM
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide derived from within residues 150 - 250 of Human DRAM.

  • Positive control
    • This antibody gave a positive signal in HeLa whole cell lysate.
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab64739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 10 µg/ml. Predicted molecular weight: 26 kDa.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Lysosomal modulator of autophagy that plays a central role in p53/TP53-mediated apoptosis. Not involved in p73/TP73-mediated autophagy.
    • Sequence similarities
      Belongs to the DRAM/TMEM150 family.
    • Cellular localization
      Lysosome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Damage regulated autophagy modulator antibody
      • Damage-regulated autophagy modulator 1 antibody
      • Damage-regulated autophagy modulator antibody
      • DNA damage-regulated autophagy modulator protein 1 antibody
      • DRAM antibody
      • dram1 antibody
      • DRAM1_HUMAN antibody
      • FLJ11259 antibody
      see all

    Images

    • Anti-DRAM antibody [M3-P4B4] (ab64739) at 10 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Performed under reducing conditions.

      Predicted band size : 26 kDa
      Observed band size : 26 kDa


      Exposure time : 20 minutes

    References

    ab64739 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you very much for confirming the details.

    I have checked the history of this antibody and can confirm that we haven't received specificity complaints for this product so I am sure protocol troubleshooting will help in getting a clean wes...

    Read More

    Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

    I have read the details you have kindly provided and would like to ask few questions in order to understand the problem better;

    ...

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (glioma stem cells)
    Specification
    glioma stem cells
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - triton-x 0.1%
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 26 2012

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (HeLa Ms Stomach Ms Kidney Ms Liver)
    Loading amount
    20 µg
    Specification
    HeLa Ms Stomach Ms Kidney Ms Liver
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
    Username

    Mr. Antibody Solutions

    Verified customer

    Submitted May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up