Anti-DRAM2 antibody (ab121554)
Key features and details
- Rabbit polyclonal to DRAM2
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DRAM2 antibody -
Description
Rabbit polyclonal to DRAM2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: ISLRVEANLH GLTLYDTAPC PINNERTRLL SRDI, corresponding to C terminal amino acids 233-266 of Human DRAM2.
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Positive control
- Human small intestine tissue; Human U 2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121554 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
Target
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Function
Induces apoptotic cell death when co-expressed with DRAM1. -
Tissue specificity
Expression is down-regulated in ovarian tumors (at protein level). Widely expressed with highest levels in placenta and heart. Not detected in brain or thymus. -
Sequence similarities
Belongs to the DRAM/TMEM150 family. -
Cellular localization
Lysosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 128338 Human
- Omim: 613360 Human
- SwissProt: Q6UX65 Human
- Unigene: 485606 Human
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Alternative names
- 2010305N14Rik antibody
- 2610318G18Rik antibody
- AI647667 antibody
see all
Images
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ab121554 at 1/100 dilution staining DRAM2 in Paraffin-embedded Human small intestine tissue by Immunohistochemistry.
Note: granular cytoplasmic positivity in glandular cells. -
ab121554 at 4 µg/ml staining DRAM2 in PFA/Triton X-100 fixed/permeabilized U2 OS cells by Immunofluorescence (green).
Note: positivity in golgi apparatus & vesicles.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121554 has not yet been referenced specifically in any publications.