Overview

  • Product nameAnti-Drebrin antibody
    See all Drebrin primary antibodies
  • Description
    Rabbit polyclonal to Drebrin
  • SpecificityAnti-Drebrin recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa).
  • Tested applicationsSuitable for: ICC, IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 22-42 of Human Drebrin.

  • Positive control
    • HeLa cell line (ab7898). Rat e16 spinal cord.
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/100.
IHC-P Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000. Predicted molecular weight: 95-120 kDa. ab11068 recognizes drebrin E/A isoforms (95 -120 kDa) and s-drebrin/A2 (approximately 45 kDa) by immunoblotting. An additional unknown band of 20 kDa may be observed in some preparations.

Target

Anti-Drebrin antibody images

  • Rabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step: Heat mediated. 1% BSA as blocking agent for 10 mins at RT. Ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation: essentially, many non-neuronal developing tissues were positive, eg: skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum. Rabbit polyclonal to Drebrin (ab11068) used in IHC(Formalin/PFA-fixed paraffin-embedded sections; Standard stABCpx-DAB detection). ab11068 detecting Drebrin protein on Rat spinal cord E16 embryo sections fixed in formaldehyde. Antigen retrieval step: Heat mediated. 1% BSA as blocking agent for 10 mins at RT. Ab11068 was used at a dilution of 1/2500 incubated for 16 hours. Secondary Antibody is biotin labeled anti rabbit IgG (1/300). Drebrin positivity conformed to published localisation: essentially, many non-neuronal developing tissues were positive, eg: skeletal primary myotubes. This picture is of spinal cord floor plate region (x20). Nuclei were counterstained using Mayer's Haemalum.
  • ab11068 staining Drebrin in mouse COR-1 cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with Triton x100 and blocking with 2% BSA was done at 250C for 15 minutes. Samples were incubated with primary antibody (1/100: in 1% BSA, 0.25% gelatin in PBS) for 3 hours at 25°C. An Alexa Fluor®488-conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

    See Abreview

  • ICC/IF image of ab11068 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11068, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Drebrin antibody (ab11068)

This product has been referenced in:
  • Xiao X  et al. N-wasp is required for structural integrity of the blood-testis barrier. PLoS Genet 10:e1004447 (2014). WB, IHC-P ; Mouse . Read more (PubMed: 24967734) »
  • Rao JS  et al. Increased neuroinflammatory and arachidonic acid cascade markers, and reduced synaptic proteins, in the postmortem frontal cortex from schizophrenia patients. Schizophr Res 147:24-31 (2013). WB ; Human . Read more (PubMed: 23566496) »

See all 7 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Mouse Cultured Cells (Cor-1, neural stem cell line)
Specification Cor-1, neural stem cell line
Fixative Paraformaldehyde
Permeabilization Yes - Triton x 0.1%
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 2% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 26 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (ovarian carcinoma)
Specification ovarian carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 19 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Chicken Tissue sections (Embryonic day 6 developing brain)
Specification Embryonic day 6 developing brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Nov 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (spinal cord)
Specification spinal cord
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6 ( not a buffer)
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 16 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (spinal cord E16 embryo)
Specification spinal cord E16 embryo
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Oct 16 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"