Recombinant Anti-Drebrin antibody [EPR12634] (ab178408)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12634] to Drebrin
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Drebrin antibody [EPR12634]
See all Drebrin primary antibodies -
Description
Rabbit monoclonal [EPR12634] to Drebrin -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Drebrin aa 100-200 (Cysteine residue). The exact sequence is proprietary.
Database link: Q16643 -
Positive control
- Jurkat, PC-12, Neuro-2a, fetal brain, SH-SY5Y and HeLa lysates; SH-SY5Y cells; fetal brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12634 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab178408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 71 kDa).
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ICC/IF |
1/100 - 1/250.
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IP |
1/10 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 71 kDa). |
ICC/IF
1/100 - 1/250. |
IP
1/10 - 1/100. |
Target
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Function
Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively. -
Tissue specificity
Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas. -
Sequence similarities
Contains 1 ADF-H domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1627 Human
- Entrez Gene: 56320 Mouse
- Entrez Gene: 81653 Rat
- Omim: 126660 Human
- SwissProt: Q16643 Human
- SwissProt: Q9QXS6 Mouse
- SwissProt: Q07266 Rat
- Unigene: 130316 Human
see all -
Alternative names
- D0S117E antibody
- DBN 1 antibody
- DBN1 antibody
see all
Images
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Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)Lanes 1 - 3: Merged signal (red and green). Green - ab178408 observed at 120 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab178408 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab178408 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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ab178408 staining Drebrin in Jurkat (human acute T cell leukemia) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol and incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Control: PBS only.
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All lanes : Anti-Drebrin antibody [EPR12634] (ab178408) at 1/1000 dilution
Lane 1 : Jurkat lysate
Lane 2 : PC-12 lysate
Lane 3 : Neuro-2a lysate
Lane 4 : Fetal brain tissue lysate
Lane 5 : SH-SY5Y cell lysate
Lane 6 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 71 kDa
Additional bands at: 120 kDa. We are unsure as to the identity of these extra bands. -
Immunoprecipitation. ab178408 at 1/1000 labeling Debrin in fetal brain lysate immunoprecipitated using ab178408 at 1/10.
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Immunofluorescent analysis of SH-SY5Y cells labeling Drebrin with ab178408 at 1/100.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab178408 has been referenced in 1 publication.
- Brezovakova V et al. Astrocytes Derived from Familial and Sporadic Alzheimer's Disease iPSCs Show Altered Calcium Signaling and Respond Differently to Misfolded Protein Tau. Cells 11:N/A (2022). PubMed: 35563735