Overview

  • Product name
    Anti-Drebrin antibody [mAbcam60932]
    See all Drebrin primary antibodies
  • Description
    Mouse monoclonal [mAbcam60932] to Drebrin
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human, Quail
  • Immunogen

    Recombinant fragment derived from within residues 450 - 550 of Human Drebrin.

  • Positive control
    • This antibody gave a positive signal in HeLa, SHSY-5Y and MALME-3M whole cell lysates, and in the following tissue lysates: Human Cortex, Rat Cortex, Rat Hippocampus, Human Melanoma. Flow Cytometry: SHSY-5Y cells
  • General notes

    This antibody clone is manufactured by Abcam.

Properties

Applications

Our Abpromise guarantee covers the use of ab60932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
  • Tissue specificity
    Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Contains 1 ADF-H domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • D0S117E antibody
    • DBN 1 antibody
    • DBN1 antibody
    • Developmentally regulated brain protein antibody
    • Developmentally-regulated brain protein antibody
    • DKFZp434D064 antibody
    • DREB_HUMAN antibody
    • Drebrin 1 antibody
    • Drebrin antibody
    • Drebrin E antibody
    • Drebrin E2 antibody
    • Drebrin1 antibody
    • DrebrinE antibody
    see all

Anti-Drebrin antibody [mAbcam60932] images

  • IHC-P image of mouse SVZ sections showing Drebrin staining using ab60932 (1/2000). The sections were fixed using Formaldehyde. Heat mediated antigen retrieval was performed using citric acid at pH6. The sections were blocked using 1% BSA for 10 mins at 21°C.

    See Abreview

  • All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 10 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutesAb60932 detects a band around 110-kDa. While this differs to its predicted molecular weight of 71-kDa, it is consistent with what has been described in the literature(PMID:10604653).
  • All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml

    Lane 1 : Human Cortex Neuronal cell lysate
    Lane 2 : Rat Cortex Neuronal cell lysate
    Lane 3 : Rat Hippocampus Tissue Lysate
    Lane 4 : Human skin tumor tissue lysate (melanoma) - total protein (ab29339)
    Lane 5 : Malme 3M (Human melanoma cells) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Predicted band size : 120 kDa
    Observed band size : 100,120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,45 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Overlay histogram showing SHSY-5Y cells stained with ab60932 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60932, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References for Anti-Drebrin antibody [mAbcam60932] (ab60932)

This product has been referenced in:
  • Sinclair LI  et al. Synaptic protein levels altered in vascular dementia. Neuropathol Appl Neurobiol 41:533-43 (2015). Read more (PubMed: 25559750) »
  • Kaczmarski W  et al. Intracellular distribution of differentially phosphorylated dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A). J Neurosci Res 92:162-73 (2014). Read more (PubMed: 24327345) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovarian carcinoma)
Specification
Ovarian carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Quail Tissue sections (e7 spinal cord)
Specification
e7 spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (E7 Spinal cord)
Specification
E7 Spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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