Overview

  • Product name
    Anti-Drebrin antibody [mAbcam60932]
    See all Drebrin primary antibodies
  • Description
    Mouse monoclonal [mAbcam60932] to Drebrin
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Chicken, Human, Quail
  • Immunogen

    Recombinant fragment derived from within residues 450 - 550 of Human Drebrin.

  • Positive control
    • This antibody gave a positive signal in HeLa, SHSY-5Y and MALME-3M whole cell lysates, and in the following tissue lysates: Human Cortex, Rat Cortex, Rat Hippocampus, Human Melanoma. Flow Cytometry: SHSY-5Y cells
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab60932 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 5 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Drebrins might play some role in cell migration, extension of neuronal processes and plasticity of dendrites, respectively.
  • Tissue specificity
    Brain neurons. Also found in the heart, placenta, skeletal muscle, kidney and pancreas.
  • Sequence similarities
    Contains 1 ADF-H domain.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • D0S117E antibody
    • DBN 1 antibody
    • DBN1 antibody
    • Developmentally regulated brain protein antibody
    • Developmentally-regulated brain protein antibody
    • DKFZp434D064 antibody
    • DREB_HUMAN antibody
    • Drebrin 1 antibody
    • Drebrin antibody
    • Drebrin E antibody
    • Drebrin E2 antibody
    • Drebrin1 antibody
    • DrebrinE antibody
    see all

Images



  • Predicted band size : 120 kDa

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: Drebrin knockout HAP1 whole cell lysate (20 µg)
    Lane 3: SH-SY5Y whole cell lysate (20 µg)

    Lanes 1 - 3: Merged signal (red and green). Green - ab60932 observed at 120 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab60932 was shown to specifically react with Drebrin in wild-type cells as signal was lost in Drebrin knockout cells. Wild-type and Drebrin knockout samples were subjected to SDS-PAGE. Ab60932 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 5 µg/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • IHC-P image of mouse SVZ sections showing Drebrin staining using ab60932 (1/2000). The sections were fixed using Formaldehyde. Heat mediated antigen retrieval was performed using citric acid at pH6. The sections were blocked using 1% BSA for 10 mins at 21°C.

    See Abreview

  • All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 120 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 10 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutesAb60932 detects a band around 110-kDa. While this differs to its predicted molecular weight of 71-kDa, it is consistent with what has been described in the literature(PMID:10604653).
  • All lanes : Anti-Drebrin antibody [mAbcam60932] (ab60932) at 10 µg/ml

    Lane 1 : Human Cortex Neuronal cell lysate
    Lane 2 : Rat Cortex Neuronal cell lysate
    Lane 3 : Rat Hippocampus Tissue Lysate
    Lane 4 : Human skin tumor tissue lysate (melanoma) - total protein (ab29339)
    Lane 5 : Malme 3M (Human melanoma cells) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution

    Predicted band size : 120 kDa
    Observed band size : 100,120 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,45 kDa,50 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • Overlay histogram showing SHSY-5Y cells stained with ab60932 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab60932, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

References

This product has been referenced in:
  • Sinclair LI  et al. Synaptic protein levels altered in vascular dementia. Neuropathol Appl Neurobiol 41:533-43 (2015). Read more (PubMed: 25559750) »
  • Kaczmarski W  et al. Intracellular distribution of differentially phosphorylated dual-specificity tyrosine phosphorylation-regulated kinase 1A (DYRK1A). J Neurosci Res 92:162-73 (2014). Read more (PubMed: 24327345) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Ovarian carcinoma)
Specification
Ovarian carcinoma
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Quail Tissue sections (e7 spinal cord)
Specification
e7 spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Apr 04 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 31 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Chicken Tissue sections (E7 Spinal cord)
Specification
E7 Spinal cord
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid pH6
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 31 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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