The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. pH 6, using a pressure boiler
Use a concentration of 1 - 4 µg/ml. The following steps are recommended prior to antibody incubation: cells fixation in ice cold 4% paraformaldehyde pH 7.2-7.3, followed by permeabilization with 0.1% Triton X-100 in PBS for 3x5 minutes.
FunctionMay play a role in cell proliferation, differentiation and death.
Tissue specificityHighest levels in skeletal muscle, heart and kidney. Low levels in colon, thymus, spleen, small intestine, lung and Leukocytes.
Sequence similaritiesBelongs to the GTP1/OBG family. Contains 1 G (guanine nucleotide-binding) domain.
Post-translational modificationsPolyubiquitinated; which induces proteolytic degradation and is impaired by interaction with RWDD1.