Overview

  • Product nameAnti-DRIL1 antibody
    See all DRIL1 primary antibodies
  • Description
    Rabbit polyclonal to DRIL1
  • SpecificityWe have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) - a portion of DRIL 1 encoded within exon 8.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionTranscription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
  • Tissue specificityWidely expressed, with highest expression in skeletal muscle, thalamus, and colon.
  • Sequence similaritiesContains 1 ARID domain.
    Contains 1 REKLES domain.
  • Cellular localizationNucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARI3A_HUMAN antibody
    • ARID domain containing 3A antibody
    • ARID domain-containing protein 3A antibody
    • ARID3A antibody
    • AT rich interactive domain 3A (BRIGHT- like) protein antibody
    • AT rich interactive domain 3A (BRIGHT-like) antibody
    • AT rich interactive domain-containing protein 3A antibody
    • AT-rich interactive domain-containing protein 3A antibody
    • B cell regulator of IgH transcription antibody
    • B-cell regulator of IgH transcription antibody
    • Bright antibody
    • dead ringer like 1 antibody
    • dead ringer, Drosophila, homolog-like 1 antibody
    • Dead ringer-like protein 1 antibody
    • DRIL1 antibody
    • DRIL3 antibody
    • DRX antibody
    • E2F binding protein 1 antibody
    • E2F-binding protein 1 antibody
    • E2FBP1 antibody
    • Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody
    see all

Anti-DRIL1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling DRIL1 with ab4445 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 62 kDa

    Detection of human DRIL 1 by Western Blot and IP using ab4445.

    WB: Lysate (100 g) from 293T cells expressing endogenous or FLAG tagged DRIL 1 (Transfected).

    IP: Lysate (+/- blocking peptide) from a 10cm plate of 293T cells.

    ab4445 used at indicated concentrations for Western Blots of endogenous DRIL 1, 1 g/ml for Western Blot of lysate from transfected cells or 10 g for IP (followed by Western Blot at 2 g/ml).

    ECL exposed for 1 second (Western Blot; IP / WB) or 1 minute (Transfected).

     

     

    Detection of human DRIL 1 by Western Blot and IP using ab4445.

    WB: Lysate (100 g) from 293T cells expressing endogenous or FLAG tagged DRIL 1 (Transfected).

    IP: Lysate (+/- blocking peptide) from a 10cm plate of 293T cells.

    ab4445 used at indicated concentrations for Western Blots of endogenous DRIL 1, 1 g/ml for Western Blot of lysate from transfected cells or 10 g for IP (followed by Western Blot at 2 g/ml).

References for Anti-DRIL1 antibody (ab4445)

This product has been referenced in:
  • Boreström C  et al. E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions. J Gen Virol 93:1065-75 (2012). WB, ChIP ; Human . Read more (PubMed: 22302879) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (lung cancer cells)
Loading amount 10000 cells
Specification lung cancer cells
Treatment RISC-free siRNA and siRNA targeting GATA2
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Submitted Dec 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"