Overview

  • Product name
  • Description
    Rabbit polyclonal to DRIL1
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla
  • Immunogen

    Synthetic peptide (Human) - a portion of DRIL 1 encoded within exon 8.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2500. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP Use at 2-10 µg/mg of lysate.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Transcription factor which may be involved in the control of cell cycle progression by the RB1/E2F1 pathway and in B-cell differentiation.
  • Tissue specificity
    Widely expressed, with highest expression in skeletal muscle, thalamus, and colon.
  • Sequence similarities
    Contains 1 ARID domain.
    Contains 1 REKLES domain.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARI3A_HUMAN antibody
    • ARID domain containing 3A antibody
    • ARID domain-containing protein 3A antibody
    • ARID3A antibody
    • AT rich interactive domain 3A (BRIGHT- like) protein antibody
    • AT rich interactive domain 3A (BRIGHT-like) antibody
    • AT rich interactive domain-containing protein 3A antibody
    • AT-rich interactive domain-containing protein 3A antibody
    • B cell regulator of IgH transcription antibody
    • B-cell regulator of IgH transcription antibody
    • Bright antibody
    • dead ringer like 1 antibody
    • dead ringer, Drosophila, homolog-like 1 antibody
    • Dead ringer-like protein 1 antibody
    • DRIL1 antibody
    • DRIL3 antibody
    • DRX antibody
    • E2F binding protein 1 antibody
    • E2F-binding protein 1 antibody
    • E2FBP1 antibody
    • Homo sapiens AT rich interactive domain 3A (BRIGHT-like) antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma tissue labelling DRIL1 with ab4445 at 1/1000 (1µg/ml). Detection: DAB.


  • Predicted band size : 62 kDa

    Detection of human DRIL 1 by Western Blot and IP using ab4445.

    WB: Lysate (100 g) from 293T cells expressing endogenous or FLAG tagged DRIL 1 (Transfected).

    IP: Lysate (+/- blocking peptide) from a 10cm plate of 293T cells.

    ab4445 used at indicated concentrations for Western Blots of endogenous DRIL 1, 1 g/ml for Western Blot of lysate from transfected cells or 10 g for IP (followed by Western Blot at 2 g/ml).

    ECL exposed for 1 second (Western Blot; IP / WB) or 1 minute (Transfected).

     

     

    Detection of human DRIL 1 by Western Blot and IP using ab4445.

    WB: Lysate (100 g) from 293T cells expressing endogenous or FLAG tagged DRIL 1 (Transfected).

    IP: Lysate (+/- blocking peptide) from a 10cm plate of 293T cells.

    ab4445 used at indicated concentrations for Western Blots of endogenous DRIL 1, 1 g/ml for Western Blot of lysate from transfected cells or 10 g for IP (followed by Western Blot at 2 g/ml).

References

This product has been referenced in:
  • Boreström C  et al. E2F1, ARID3A/Bright and Oct-2 factors bind to the Epstein-Barr virus C promoter, EBNA1 and oriP, participating in long-distance promoter-enhancer interactions. J Gen Virol 93:1065-75 (2012). WB, ChIP ; Human . Read more (PubMed: 22302879) »

See 1 Publication for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (lung cancer cells)
Loading amount
10000 cells
Specification
lung cancer cells
Treatment
RISC-free siRNA and siRNA targeting GATA2
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Dec 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up