Overview

  • Product name
  • Description
    Rabbit polyclonal to DRIM
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Cow, Chimpanzee, Rhesus monkey, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 2735 and 2785 of human DRIM (NP_055318.2)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab86093 was affinity purified using an epitope specific to DRIM immobilized on solid support.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 318 kDa (predicted molecular weight: 318 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Relevance
    DRIM, also known as UTP20, is a component of the U3 small nucleolar RNA (snoRNA) protein complex (U3 snoRNP) and is involved in 18S rRNA processing. DRIM contains 2 HEAT repeats.
  • Cellular localization
    Nucleus; nucleolus
  • Database links
  • Alternative names
    • 3830408P06Rik antibody
    • AA617408 antibody
    • Down regulated in metastasis antibody
    • Drim antibody
    • mDRIM antibody
    • mDRIN antibody
    • Novel nucleolar protein 73 antibody
    • Protein Key-1A6 antibody
    • RGD1560606 antibody
    • Small subunit processome component 20 homolog antibody
    • UTP20, small subunit (SSU) processome component, homolog (yeast) antibody
    see all

Images

  • All lanes : Anti-DRIM antibody (ab86093) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 318 kDa
    Observed band size : 318 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of DRIM by Western Blot of Immunprecipitate.
    ab86093 at 1µg/ml staining DRIM in HeLa whole cell lysate immunoprecipitated using ab86093 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab86093 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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