The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIP: Use at 1 - 4 µg/mg of lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionRequired for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
Sequence similaritiesBelongs to the Mediator complex subunit 23 family.