Overview

  • Product nameAnti-DRIP130 antibody
    See all DRIP130 primary antibodies
  • Description
    Rabbit polyclonal to DRIP130
  • Tested applicationsSuitable for: WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rabbit, Dog, Rhesus monkey, Gorilla, Bat
  • Immunogen

    Synthetic peptide, corresponding to a region between residues 1300 and the C-terminus (residue 1368) of Human DRIP130 (Swiss-Prot entry Q9ULK4)

  • Positive control
    • Nuclear extract from HeLa cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab70450 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 150 kDa (predicted molecular weight: 157 kDa).
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionRequired for transcriptional activation subsequent to the assembly of the preinitiation complex (By similarity). Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for transcriptional activation by adenovirus E1A protein. Required for ELK1-dependent transcriptional activation in response to activated Ras signaling.
    • Sequence similaritiesBelongs to the Mediator complex subunit 23 family.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 130 kDa transcriptional co activator antibody
      • 133 kDa transcriptional co activator antibody
      • Activator recruited cofactor 130 kDa component antibody
      • Activator-recruited cofactor 130 kDa component antibody
      • ARC 130 antibody
      • ARC130 antibody
      • Cofactor required for Sp1 transcriptional activation subunit 3 130kDa antibody
      • Cofactor required for Sp1 transcriptional activation subunit 3 130kDa mediator antibody
      • Cofactor required for Sp1 transcriptional activation subunit 3 antibody
      • CRSP 130 antibody
      • CRSP 130 kD subunit antibody
      • CRSP 133 antibody
      • CRSP 3 antibody
      • CRSP complex subunit 3 antibody
      • CRSP130 antibody
      • CRSP133 antibody
      • CRSP3 antibody
      • CRSP3 protein antibody
      • DKFZp434H0117 antibody
      • DRIP 130 antibody
      • DRIP130 antibody
      • hSur-2 antibody
      • KIAA1216 antibody
      • MED 23 antibody
      • med23 antibody
      • MED23_HUMAN antibody
      • Mediator complex subunit 23 antibody
      • Mediator of RNA polymerase II transcription subunit 23 antibody
      • MRT18 antibody
      • Protein sur-2 homolog antibody
      • SUR 2 antibody
      • SUR2 antibody
      • Transcriptional co activator CRSP 130 antibody
      • Transcriptional co activator CRSP130 antibody
      • Transcriptional coactivator CRSP130 antibody
      • Vitamin D3 receptor interacting protein antibody
      • Vitamin D3 receptor interacting protein complex 130 kDa component antibody
      • Vitamin D3 receptor-interacting protein complex 130 kDa component antibody
      see all

    Anti-DRIP130 antibody images

    • All lanes : Anti-DRIP130 antibody (ab70450) at 0.33 µg/ml

      Lane 1 : Nuclear extract from HeLa cells. at 5 µg
      Lane 2 : Nuclear extract from HeLa cells. at 25 µg

      Developed using the ECL technique

      Predicted band size : 157 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)


      Exposure time : 5 minutes
    • Anti-DRIP130 antibody (ab70450) at 1/2000 dilution + HEK293 whole cell lysate at 5 µg

      Secondary
      undiluted HRP-conjugated goat anti-rabbit polyclonal IgG
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 157 kDa
      Observed band size : 150 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 90 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 1 minute

      This image is courtesy of an anonymous Abreview

      See Abreview

    References for Anti-DRIP130 antibody (ab70450)

    This product has been referenced in:
    • Meyer KD  et al. Cooperative activity of cdk8 and GCN5L within Mediator directs tandem phosphoacetylation of histone H3. EMBO J 27:1447-57 (2008). Read more (PubMed: 18418385) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (293)
    Loading amount 5 µg
    Specification 293
    Gel Running Conditions Reduced Denaturing (6)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

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    Verified customer

    Submitted Oct 15 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"