Overview

  • Product nameAnti-Drosha antibody
    See all Drosha primary antibodies
  • Description
    Rabbit polyclonal to Drosha
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region between residue 1050 and 1100 of human Drosha (NP_037367.3)

  • Positive control
    • Human Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma tissues and Mouse Teratoma tissue.
  • General notes

    Concentration is optimized for IHC and not determined

Properties

Applications

Our Abpromise guarantee covers the use of ab85027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • FunctionRibonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesContains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 2 RNase III domains.
  • DomainThe 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
  • Cellular localizationNucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • DROSHA antibody
    • Drosha double stranded RNA specific endoribonuclease antibody
    • Drosha ribonuclease type III antibody
    • Etohi2 antibody
    • HSA242976 antibody
    • Nuclear RNase III Drosha antibody
    • p241 antibody
    • Protein Drosha antibody
    • Putative protein p241 which interacts with transcription factor Sp1 antibody
    • Putative ribonuclease III antibody
    • RANSE3L antibody
    • Ribonuclease 3 antibody
    • Ribonuclease III antibody
    • Ribonuclease III nuclear antibody
    • Ribonuclease type III nuclear antibody
    • RibonucleaseIII antibody
    • RN 3 antibody
    • RN3 antibody
    • RNase 3 antibody
    • RNase III antibody
    • RNase3 antibody
    • RNASE3L antibody
    • RNaseIII antibody
    • RNASEN antibody
    • RNC_HUMAN antibody
    see all

Anti-Drosha antibody images

  • ab85027 at 1/250 dilution staining Drosha in human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
  • ab85027 at 1/250 dilution staining Drosha in mouse teratoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
  • ab85027 at 1/250 dilution staining Drosha in human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
  • All lanes : Anti-Drosha antibody (ab85027) at 1/500 dilution

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 145 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • ICC/IF image of ab85027 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85027, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Drosha antibody (ab85027)

ab85027 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. I am sorry that ab85027 is detecting an incorrect localization of Drosha in IHC. I agree that your protocol should be producing a clearer nuclear stain with less background. For our records, did you run a no-primary antibod...

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Thank you for your recent contact and your kind reminder.
I have checked our internal system which stores all the in-coming and out-going messages. According to our CRM system, your enquiry was received in February (Tue 21 Feb 12) and I replied to...

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Thank you for your response.
ICC stands for for immuno-cytochemistry which requires cells (not tissues); whilst IHC is the abbreviation for immuno-histochemistry.
If you wish to use tissue sections of human gastric samples then you need an an...

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Thank you for your response.
Both antibodies (ab58589 and ab85027) are suitable for IHC-P application and they can detect human Drosha. I would suggest taking a look at the on-line product datasheets to see which one would suit your need best. You...

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Thank you for your enquiry regarding ab12286.
Currently, we have 12 specific publications on this product (ab12286) and the list of these papers can be viewed on the on the on-line datasheet. Since ab12286 has been tested in ICC/IF, IP, WB, ICC, C...

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Thank you for your enquiry. Currently, we only sell polyclonal antibodies against Drosha. Is there any specific reason why you are interested in monoclonal rabbit antibodies? We have 2 rabbit polyclonal antibodies: ab12286 and ab85027 and one goat ...

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We do not give the precise concentration of IgG in this product. ab85027 is prepared to provide specific IHC staining within the recommended range of dilutions.  The concentration of IgG is less than or equal to 1 mg/ml.  For your purposes where you wi...

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Thank you for your patience. The antibody concentration for 0.1 ml was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG, thus the concentration would be 10 mg/ml. I am confirming this still with the lab. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"