Recombinant
RabMAb

Anti-Drosha antibody [EPR12794] - BSA and Azide free (ab227460)

Overview

  • Product name
    Anti-Drosha antibody [EPR12794] - BSA and Azide free
    See all Drosha primary antibodies
  • Description
    Rabbit monoclonal [EPR12794] to Drosha - BSA and Azide free
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Drosha aa 1-100. The exact sequence is proprietary.
    Database link: Q9NRR4

  • Positive control
    • Jurkat, 293 and HeLa cell lysates; Human lung adenocarcinoma tissue; HeLa cells.
  • General notes

    ab227460 is a PBS only buffer version of ab183732 containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab183732 for information on dilutions, protocols, and image data.

Properties

Applications

Our Abpromise guarantee covers the use of ab227460 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376-Rabbit monoclonal IgG (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 159 kDa (predicted molecular weight: 159 kDa).
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 1 DRBM (double-stranded RNA-binding) domain.
    Contains 2 RNase III domains.
  • Domain
    The 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
  • Cellular localization
    Nucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.
  • Information by UniProt
  • Database links
  • Alternative names
    • DROSHA antibody
    • Drosha double stranded RNA specific endoribonuclease antibody
    • Drosha ribonuclease type III antibody
    • Etohi2 antibody
    • HSA242976 antibody
    • Nuclear RNase III Drosha antibody
    • p241 antibody
    • Protein Drosha antibody
    • Putative protein p241 which interacts with transcription factor Sp1 antibody
    • Putative ribonuclease III antibody
    • RANSE3L antibody
    • Ribonuclease 3 antibody
    • Ribonuclease III antibody
    • Ribonuclease III nuclear antibody
    • Ribonuclease type III nuclear antibody
    • RibonucleaseIII antibody
    • RN 3 antibody
    • RN3 antibody
    • RNase 3 antibody
    • RNase III antibody
    • RNase3 antibody
    • RNASE3L antibody
    • RNaseIII antibody
    • RNASEN antibody
    • RNC_HUMAN antibody
    see all

Images

  • This IHC data was generated using the same anti-Drosha antibody clone, EPR12794, in a different buffer formulation (cat# ab183732).

    Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling Drosha with ab183732 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

References

This product has been referenced in:
  • Silveyra P  et al. Knockdown of Drosha in human alveolar type II cells alters expression of SP-A in culture: a pilot study. Exp Lung Res 40:354-66 (2014). WB ; Human . Read more (PubMed: 25058539) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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