Anti-Drosophila FMR1 antibody [6A15] (ab10299)

Overview

  • Product name
    Anti-Drosophila FMR1 antibody [6A15]
  • Description
    Mouse monoclonal [6A15] to Drosophila FMR1
  • Tested applications
    Suitable for: IP, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
    Does not react with: Human
  • Immunogen

    His-dFMR1 fusion protein (Drosophilia melanogaster) (C-terminal 580aa).

  • General notes


    Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Protein A purified from tissue culture supernatant.
  • Primary antibody notes
    Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway.
  • Clonality
    Monoclonal
  • Clone number
    6A15
  • Myeloma
    Sp2/0
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 25 µg/mg of lysate. See Abreview.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 85 kDa (predicted molecular weight: 82 kDa).

Target

  • Relevance
    Drosophila FMR1 is a RNA-binding protein that associates with translating ribosomes and acts as a negative translational regulator of specific mRNAs. Represses translation of futsch to regulate microtubule-dependent synaptic growth and function. Part of the RNA interference (RNAi)-related apparatus; double-stranded RNA induces potent and specific gene silencing. Regulates photoreceptor structure and neurotransmission in the eye. Required for stability of the central pair of microtubules in the spermatid axoneme
  • Cellular localization
    Cytoplasmic
  • Database links
    • Alternative names
      • dFMR antibody
      • dFMR1 antibody
      • dFXR antibody
      • Drosophila fragile X mental retardation protein antibody
      • fragile X mental retardation protein antibody
      see all

    Anti-Drosophila FMR1 antibody [6A15] images

    • ab10299 used to Western blot specific immunoprecipitates. In this experiment tissue lysate from Drosophila cellularizing embryos were immunoprecipitated with either ab10299 or and anti-FLAG antibody (at 25ug/mg lysate). Supernatant and pellet from these immunoprecipitates were then Western blotted with ab10299. The image clearly shows that ab10299 brings down dFMRP in the immunoprecipitation pellet whilst the anti-FLAG monoclonal does not.

      See Abreview

    References for Anti-Drosophila FMR1 antibody [6A15] (ab10299)

    This product has been referenced in:
    • Ahmad M  et al. RNA topoisomerase is prevalent in all domains of life and associates with polyribosomes in animals. Nucleic Acids Res 44:6335-49 (2016). WB ; Drosophila melanogaster . Read more (PubMed: 27257063) »
    • Novak SM  et al. Regulation of Heart Rate in Drosophila via Fragile X Mental Retardation Protein. PLoS One 10:e0142836 (2015). WB . Read more (PubMed: 26571124) »

    See all 9 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunoprecipitation
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - other (cellularizing embryos, 10,000 x g supernatant)
    Total protein in input
    15 µg
    Specification
    cellularizing embryos, 10,000 x g supernatant
    Immuno-precipitation step
    Protein G
    Username

    Dr. Ophelia Papoulas

    Verified customer

    Submitted May 19 2006

    Thanks for your enquiry. The immunogen used to generate this antibody was a His-dFMR1 fusion protein (Drosophilia melanogaster) (amino-terminal 580aa). It is not available for purchase. If you have any additional questions, please let us know.

    Thank you for your enquiry. The originator used extracts of Drosophila S2 tissue culture cells. That's all we know.

    Thank you for your enquiry. The originator used extracts of Drosophila S2 tissue culture cells. That's all we know.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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