Anti-Drosophila FMR1 antibody [6A15] (ab10299)

Overview

  • Product nameAnti-Drosophila FMR1 antibody [6A15]
  • Description
    Mouse monoclonal [6A15] to Drosophila FMR1
  • Tested applicationsSuitable for: IP, ICC/IF, ELISA, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
    Does not react with: Human
  • Immunogen

    His-dFMR1 fusion protein (Drosophilia melanogaster) (C-terminal 580aa).

  • General notes


    Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesProtein A purified from tissue culture supernatant.
  • Primary antibody notesFragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway.
  • ClonalityMonoclonal
  • Clone number6A15
  • MyelomaSp2/0
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab10299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 25 µg/mg of lysate. See Abreview.
ICC/IF Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 85 kDa (predicted molecular weight: 82 kDa).

Target

  • RelevanceDrosophila FMR1 is a RNA-binding protein that associates with translating ribosomes and acts as a negative translational regulator of specific mRNAs. Represses translation of futsch to regulate microtubule-dependent synaptic growth and function. Part of the RNA interference (RNAi)-related apparatus; double-stranded RNA induces potent and specific gene silencing. Regulates photoreceptor structure and neurotransmission in the eye. Required for stability of the central pair of microtubules in the spermatid axoneme
  • Cellular localizationCytoplasmic
  • Database links
    • Alternative names
      • dFMR antibody
      • dFMR1 antibody
      • dFXR antibody
      • Drosophila fragile X mental retardation protein antibody
      • fragile X mental retardation protein antibody
      see all

    Anti-Drosophila FMR1 antibody [6A15] images

    • ab10299 used to Western blot specific immunoprecipitates. In this experiment tissue lysate from Drosophila cellularizing embryos were immunoprecipitated with either ab10299 or and anti-FLAG antibody (at 25ug/mg lysate). Supernatant and pellet from these immunoprecipitates were then Western blotted with ab10299. The image clearly shows that ab10299 brings down dFMRP in the immunoprecipitation pellet whilst the anti-FLAG monoclonal does not.

      See Abreview

    References for Anti-Drosophila FMR1 antibody [6A15] (ab10299)

    This product has been referenced in:
    • Sudhakaran IP  et al. FMRP and Ataxin-2 function together in long-term olfactory habituation and neuronal translational control. Proc Natl Acad Sci U S A 111:E99-E108 (2014). Drosophila melanogaster . Read more (PubMed: 24344294) »
    • Callan MA  et al. Fragile X Protein is required for inhibition of insulin signaling and regulates glial-dependent neuroblast reactivation in the developing brain. Brain Res 1462:151-61 (2012). ICC/IF ; Drosophila melanogaster . Read more (PubMed: 22513101) »

    See all 7 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - other (cellularizing embryos, 10,000 x g supernatant)
    Total protein in input 15 µg
    Specification cellularizing embryos, 10,000 x g supernatant
    Immuno-precipitation step Protein G
    Username

    Dr. Ophelia Papoulas

    Verified customer

    Submitted May 19 2006

    Thanks for your enquiry. The immunogen used to generate this antibody was a His-dFMR1 fusion protein (Drosophilia melanogaster) (amino-terminal 580aa). It is not available for purchase. If you have any additional questions, please let us know.

    Thank you for your enquiry. The originator used extracts of Drosophila S2 tissue culture cells. That's all we know.

    Thank you for your enquiry. The originator used extracts of Drosophila S2 tissue culture cells. That's all we know.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"