Overview

  • Product nameAnti-DRP1 antibody
    See all DRP1 primary antibodies
  • Description
    Rabbit polyclonal to DRP1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Saccharomyces cerevisiae, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 107-156 (IRQEIENETE RISGNNKGVS PEPIHLKIFS PNVVNLTLVD LPGMTKVPVG) of Human DNM1L (NP_036192).

  • Positive control
    • Hela cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab118926 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 82 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF Use a concentration of 2 µg/ml.

Target

  • FunctionFunctions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
    Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
  • Tissue specificityUbiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
  • Involvement in diseaseNote=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
  • Sequence similaritiesBelongs to the dynamin family.
    Contains 1 GED domain.
  • DomainThe GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
  • Post-translational
    modifications
    Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
    Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
    S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
    Ubiquitination by MARCH5 affects mitochondrial morphology.
  • Cellular localizationCytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • DLP1 antibody
    • dnm1l antibody
    • DNM1L_HUMAN antibody
    • Dnm1p/Vps1p-like protein antibody
    • dnml1 antibody
    • DRP1 antibody
    • DVLP antibody
    • Dymple antibody
    • Dynamin 1 like antibody
    • Dynamin family member proline-rich carboxyl-terminal domain less antibody
    • Dynamin like protein antibody
    • Dynamin related protein 1 antibody
    • Dynamin-1-like protein antibody
    • Dynamin-like protein 4 antibody
    • Dynamin-like protein antibody
    • Dynamin-like protein IV antibody
    • Dynamin-related protein 1 antibody
    • DYNIV 11 antibody
    • EMPF antibody
    • EMPF1 antibody
    • FLJ41912 antibody
    • HdynIV antibody
    • VPS1 antibody
    see all

Anti-DRP1 antibody images

  • Anti-DRP1 antibody (ab118926) at 1 µg/ml + Hela cell lysate at 10 µg

    Predicted band size : 82 kDa
  • Immunofluorescence analysis of Mouse brain stem cells staining DNM1L with ab118926 at 2 µg/ml.

References for Anti-DRP1 antibody (ab118926)

ab118926 has not yet been referenced specifically in any publications.

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