Overview

  • Product nameAnti-DRP1 antibody
    See all DRP1 primary antibodies
  • Description
    Rabbit polyclonal to DRP1
  • Tested applicationsSuitable for: WB, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Hamster, Monkey
  • Immunogen

    A synthetic peptide made to an N-terminal region within residues 1-100 of the human DNM1L.

  • Positive control
    • Human kidney lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab54038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 81 kDa (predicted molecular weight: 82 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 25208467
IHC-FoFr Use at an assay dependent concentration. PubMed: 20660112

Target

  • FunctionFunctions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
    Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
  • Tissue specificityUbiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
  • Involvement in diseaseNote=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
  • Sequence similaritiesBelongs to the dynamin family.
    Contains 1 GED domain.
  • DomainThe GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
  • Post-translational
    modifications
    Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
    Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
    S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
    Ubiquitination by MARCH5 affects mitochondrial morphology.
  • Cellular localizationCytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • DLP1 antibody
    • dnm1l antibody
    • DNM1L_HUMAN antibody
    • Dnm1p/Vps1p-like protein antibody
    • dnml1 antibody
    • DRP1 antibody
    • DVLP antibody
    • Dymple antibody
    • Dynamin 1 like antibody
    • Dynamin family member proline-rich carboxyl-terminal domain less antibody
    • Dynamin like protein antibody
    • Dynamin related protein 1 antibody
    • Dynamin-1-like protein antibody
    • Dynamin-like protein 4 antibody
    • Dynamin-like protein antibody
    • Dynamin-like protein IV antibody
    • Dynamin-related protein 1 antibody
    • DYNIV 11 antibody
    • EMPF antibody
    • EMPF1 antibody
    • FLJ41912 antibody
    • HdynIV antibody
    • VPS1 antibody
    see all

Anti-DRP1 antibody images

  • All lanes : Anti-DRP1 antibody (ab54038) at 2 µg/ml

    Lane 1 : Drp1-mut
    Lane 2 : OPA1-null
    Lane 3 : Double mut
    Lane 4 : Mfn2-null
    Lane 5 : Mfn1-null
    Lane 6 : Wildtype.


    Predicted band size : 82 kDa

References for Anti-DRP1 antibody (ab54038)

This product has been referenced in:
  • Ota K  et al. Relocation of p25¿/tubulin polymerization promoting protein from the nucleus to the perinuclear cytoplasm in the oligodendroglia of sporadic and COQ2 mutant multiple system atrophy. Acta Neuropathol Commun 2:136 (2014). IHC-P ; Human . Read more (PubMed: 25208467) »
  • Yano H  et al. Inhibition of mitochondrial protein import by mutant huntingtin. Nat Neurosci 17:822-31 (2014). ICC/IF ; Mouse . Read more (PubMed: 24836077) »

See all 3 Publications for this product

Product Wall

Thank you for your email. I am sorry to hear this product is not working in mouse samples. Would it be possible for you to submit an Abreview of your experiences with this product? This will help other users. I would be happy to refund the prod...

Read More
Application Western blot
Sample Human Cell lysate - whole cell (liver)
Loading amount 20 µg
Specification liver
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Username

Dr. Maaike Kockx

Verified customer

Submitted Sep 14 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"