Overview

  • Product nameAnti-DRP1 antibody
    See all DRP1 primary antibodies
  • Description
    Mouse monoclonal to DRP1
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Recombinant full length protein, corresponding to amino acids 1-711 of Human DNM1L

Properties

Applications

Our Abpromise guarantee covers the use of ab56788 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 82 kDa.
IHC-P Use a concentration of 1 µg/ml.
ICC/IF 1/200.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • FunctionFunctions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis-dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport.
    Isoform 1 and isoform 4 inhibit peroxisomal division when overexpressed.
  • Tissue specificityUbiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons.
  • Involvement in diseaseNote=May be associated with Alzheimer disease through beta-amyloid-induced increased S-nitrosylation of DNM1L, which triggers, directly or indirectly, excessive mitochondrial fission, synaptic loss and neuronal damage.
  • Sequence similaritiesBelongs to the dynamin family.
    Contains 1 GED domain.
  • DomainThe GED domain folds back to interact, in cis, with the GTP-binding domain and middle domain, and interacts, in trans, with the GED domains of other DNM1L molecules, and is thus critical for activating GTPase activity and for DNM1L dimerization.
  • Post-translational
    modifications
    Phosphorylation/dephosphorylation events on two sites near the GED domain regulate mitochondrial fission. Phosphorylation on Ser-637 inhibits mitochondrial fissin probably through preventing intramolecular interaction. Dephosphorylated on this site by PPP3CA which promotes mitochondrial fission. Phosphorylation on Ser-616 also promotes mitochondrial fission.
    Sumoylated on various lysine residues within the B domain. Desumoylated by SENP5 during G2/M transition of mitosis. Appears to be linked to its catalytic activity.
    S-nitrosylation increases DNM1L dimerization, mitochondrial fission and causes neuronal damage.
    Ubiquitination by MARCH5 affects mitochondrial morphology.
  • Cellular localizationCytoplasm > cytosol. Golgi apparatus. Endomembrane system. Mainly cytosolic. Translocated to the mitochondrial membrane through interaction with FIS1. Colocalized with MARCH5 at mitochondrial membrane. Localizes to mitochondria at sites of division. Associated with peroxisomal membranes, partly recruited there by PEX11B. May also be associated with endoplasmic reticulum tubules and cytoplasmic vesicles and found to be perinuclear. In some cell types, localizes to the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • DLP1 antibody
    • dnm1l antibody
    • DNM1L_HUMAN antibody
    • Dnm1p/Vps1p-like protein antibody
    • dnml1 antibody
    • DRP1 antibody
    • DVLP antibody
    • Dymple antibody
    • Dynamin 1 like antibody
    • Dynamin family member proline-rich carboxyl-terminal domain less antibody
    • Dynamin like protein antibody
    • Dynamin related protein 1 antibody
    • Dynamin-1-like protein antibody
    • Dynamin-like protein 4 antibody
    • Dynamin-like protein antibody
    • Dynamin-like protein IV antibody
    • Dynamin-related protein 1 antibody
    • DYNIV 11 antibody
    • EMPF antibody
    • EMPF1 antibody
    • FLJ41912 antibody
    • HdynIV antibody
    • VPS1 antibody
    see all

Anti-DRP1 antibody images

  • DNM1L was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Mouse monoclonal to DNM1L and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab56788.
    Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
    Band: 90kDa; DNM1L.


  • Predicted band size : 82 kDa

    Image from PLoS Pathog. 2010 Jul; 6(7): e1001012. Fig 9E, doi: 10.1371/journal.ppat.1001012

    HeLa cells were transfected with N.C. siRNA or hDRP1-targeted siRNA (#1–#3) for 72 h, and the knockdown of endogenous DRP1 was analyzed by Western blotting using anti-DRP1 antibody ab56788. 

  • ab56788 staining overexpressed Human DNM1L in the HEK293 cell line by ICC/IF(Immunocytochemistry/immunofluorescence). Cells were fixed with 4% Paraformaldehyde, permeabilized with Triton X-100 1% and blocked with 5% Serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/200 in 5% Horse serum) for 2 hours. An Alexa Fluor®488-conjugated Goat anti-mouse IgG polyclonal(1/400) was used as the secondary antibody. Results showed that overexpressed hDNM1L co-localizes with mitochondria.

    See Abreview

  • Anti-DRP1 antibody (ab56788) at 1/500 dilution + Fly heart tube Lysate infected with Drosophila C Virus at 10 µg

    Secondary
    HRP conjugated Goat anti-mouse IgG at 1/4000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 82 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    Blocked using 5% Milk for 1 hour.

    See Abreview

  • DNM1L antibody (ab56788) used in immunohistochemistry at 1ug/ml on formalin fixed and paraffin embedded human leiomyosarcoma tissue.
  • Overlay histogram showing HEK293 cells stained with ab56788 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56788, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-DRP1 antibody (ab56788)

This product has been referenced in:
  • Kitaoka Y  et al. Combined effects of resistance training and calorie restriction on mitochondrial fusion and fission proteins in rat skeletal muscle. J Appl Physiol (1985) 121:806-810 (2016). Read more (PubMed: 27539498) »
  • Jung JU  et al. NIK/MAP3K14 Regulates Mitochondrial Dynamics and Trafficking to Promote Cell Invasion. Curr Biol N/A:N/A (2016). Human . Read more (PubMed: 27889261) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample Bird Tissue lysate - whole (Pectoralis muscle)
Gel Running Conditions Reduced Denaturing (7.5% acrylamide)
Loading amount 20 µg
Treatment Homogenized in Tris (5mM) / EGTA (2mM) solution
Specification Pectoralis muscle
Blocking step Milk as blocking agent for 1 hour(s) and 15 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Oct 10 2016

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Total protein in input 1e+007 cells
Immuno-precipitation step Other - Protein G Dynabeads
Specification A549 (human lung epithelium cells)
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Abcam user community

Verified customer

Submitted Jul 12 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample African Green Monkey Cell lysate - whole cell (VERO E6)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 75000 cells
Specification VERO E6
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Jun 28 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Tissue lysate - whole (A549 (human lung epithelium cells))
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 75000 cells
Specification A549 (human lung epithelium cells)
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jun 28 2016

Application Immunoprecipitation
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts (MEFs))
Total protein in input 2e+006 cells
Immuno-precipitation step Other - Protein G Dynabeads
Specification Mouse embryonic fibroblasts (MEFs)
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Submitted May 13 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (mouse embryonic fibroblasts)
Gel Running Conditions Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount 50000 cells
Specification mouse embryonic fibroblasts
Blocking step Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Mar 29 2016

Application Western blot
Sample Mouse Tissue lysate - whole (Brain, liver, kidney)
Gel Running Conditions Reduced Denaturing (4-15% gradient gel)
Loading amount 20 µg
Specification Brain, liver, kidney
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Shiran Udi

Verified customer

Submitted Nov 20 2015

Application Western blot
Sample Human Cell lysate - whole cell (Primary fibroblasts)
Gel Running Conditions Reduced Denaturing (4-12% NuPAGE gel)
Loading amount 20 µg
Specification Primary fibroblasts
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Ms. Jenny Sharpe

Verified customer

Submitted Jun 19 2015

Thank you for your enquiry and your interest in our products.

I can confirm that ab56788 has not been tested for cross-reactivity with rabbit samples. According to the Blast search, the homology between human and rabbit the immunogen sequence...

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Thank you for contacting us. We have not tested ab56788 in immunocytochemistry (ICC/IF) for cell staining, however we have tested it in paraffin-embedded tissues sections (IHC-P) using chromogenic detection.  We would not be able to guarantee t...

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