Anti-ds DNA antibody [11B6] (ab418)

Overview

  • Product name
    Anti-ds DNA antibody [11B6]
    See all ds DNA primary antibodies
  • Description
    Mouse monoclonal [11B6] to ds DNA
  • Specificity
    In ELISA : This antibody shows 100% reactivity to calf dsDNA and 5% to calf ssDNA. It does not react with RNA.
  • Tested applications
    Suitable for: ELISAmore details
  • General notes
    This antibody can only be used on highly purified dsDNA that has been stripped of chromatin. Histones can be removed by using an incubation step with proteinase K. We also recommend that you dilute no more than 1:10 - also try a 1:5 dilution.

Properties

  • Form
    Lyophilised:Reconstitute with 1ml sterile water.
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    11B6
  • Myeloma
    unknown
  • Isotype
    IgM
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5 - 1/10. We recommend that you dilute no more than 1/10 - also try a 1/5 dilution.

Target

  • Relevance
    dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.
  • Cellular localization
    Nuclear
  • Alternative names
    • Double stranded deoxyribonucleic acid antibody
    • Double stranded DNA antibody
    • dsDNA antibody

References

ab418 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.

Please check the following primary antibody datasheets

http://www.abcam.com/ds-DNA-antibody-HYB331-01-ab27156.html

http://www.abcam.com/ds-DNA-antibody-11B6-ab418.html

The compatible seco...

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Thank you for contacting us. Please check the datasheet for anti DNA antibodies ab27156; http://www.abcam.com/ds-DNA-antibody-HYB331-01-ab27156.html ab418; http://www.abcam.com/ds-DNA-antibody-11B6-ab418.html Please check the compatible s...

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Thank you for your call this week and for your patience while I have been in touch with the lab regarding ab418. This antibody has not been specifically tested against plasmids, however it should recognize double-stranded DNA free of chromatin...

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Thank you for your enquiry. I can tell you that the testing of this antibody was performed by immunohistochemistry using acetone fixed Hep2 and Hela cells. Unfortunately I do not have further details as to where this antibody has been applied on LR ...

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This antibody will work on DNA measurement (by Elisa method).

All the information available for this product is listed on the on-line datasheet (price, datasheet, publication, suitability, cross-reactivity). We will update the on-line datasheet of this product as soon as get more data.

We would recommend trying 1 in 10, 1 in 50 and 1 in 100 in ELISA since every study is different. Please note that the antibody will not react aganst chromatin so make sure that your DNA is protein free.

Because it is tissue culture supernatant the concentration is difficult to determine, but we estimate it is approx. 0.1mg/ml.

10 base pairs

This antibody has been used in immunhistochemistry and ELISA applications. We do not have any reports of it being used for purification of double stranded DNA, though it may be suitable for this purpose.

1-10 of 14 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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