Anti-DsbA L antibody (ab92819)
Key features and details
- Rabbit polyclonal to DsbA L
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
-
Product name
Anti-DsbA L antibody -
Description
Rabbit polyclonal to DsbA L -
Host species
Rabbit -
Specificity
ab92819 is specific for the ~25 kDa DsbA-L protein in Western blots of 3T3-L1 adipocytes and mouse white and brown adipose tissue lysate samples. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant full length Mouse DsbA L protein.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 40% Glycerol, Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92819 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000. Detects a band of approximately 25 kDa.
|
Notes |
---|
WB
1/2000. Detects a band of approximately 25 kDa. |
Target
-
Relevance
Disulfide-bond-A oxidoreductase-like protein (DsbA-L, previously named as GST Kappa) is an adiponectin-interacting protein. DsbA-L is highly expressed in adipose tissue, and its expression level is negatively correlated with obesity in mice and humans. DsbA-L expression in 3T3-L1 adipocytes is stimulated by the insulin sensitizer rosiglitazone and inhibited by the inflammatory cytokine TNFalpha. Polymorphism of DsbA-L gene has recently been implicated in insulin secretion and body fat distribution. Overexpression of DsbA-L promotes adiponectin multimerization while suppressing DsbA-L expression by RNAi markedly and selectively reduces adiponectin levels and secretion in 3T3-L1 adipocytes. Recent studies identify DsbA-L as a key regulator for adiponectin biosynthesis. -
Alternative names
- DSBA like thioredoxin domain containing protein antibody
Images
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (5)
ab92819 has been referenced in 5 publications.
- Harris NJ et al. Structure formation during translocon-unassisted co-translational membrane protein folding. Sci Rep 7:8021 (2017). WB . PubMed: 28808343
- Liu X et al. Chinese herb extract improves liver steatosis by promoting the expression of high molecular weight adiponectin in NAFLD rats. Mol Med Rep 16:5580-5586 (2017). PubMed: 28849192
- Jedrychowski MP et al. Adiporedoxin, an upstream regulator of ER oxidative folding and protein secretion in adipocytes. Mol Metab 4:758-70 (2015). PubMed: 26629401
- Fu Z et al. Dietary ?-3 polyunsaturated fatty acids decrease retinal neovascularization by adipose-endoplasmic reticulum stress reduction to increase adiponectin. Am J Clin Nutr 101:879-88 (2015). PubMed: 25833984
- Walker GE et al. Pediatric obesity and vitamin D deficiency: a proteomic approach identifies multimeric adiponectin as a key link between these conditions. PLoS One 9:e83685 (2014). WB . PubMed: 24404137