Overview

  • Product nameAnti-Dscam antibody
    See all Dscam primary antibodies
  • Description
    Rabbit polyclonal to Dscam
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Fruit fly (Drosophila melanogaster)
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1150 - 1250 of Fruit fly (Drosophila melanogaster) Dscam.

    (Peptide available as ab43846.)

  • Positive control
    • This antibody gave a positive signal in Drosophila S2 cell lysate.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab43847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 222 kDa.Can be blocked with Human Dscam peptide (ab43846).

Target

  • FunctionCell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38.
  • Tissue specificityPrimarily expressed in brain.
  • Sequence similaritiesContains 6 fibronectin type-III domains.
    Contains 10 Ig-like C2-type (immunoglobulin-like) domains.
  • DomainIg-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC.
  • Post-translational
    modifications
    Phosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin.
  • Cellular localizationSecreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.
  • Information by UniProt
  • Database links
    • Alternative names
      • CHD2 42 antibody
      • CHD2 52 antibody
      • CHD2 antibody
      • CHD2-52 antibody
      • Down syndrome cell adhesion molecule antibody
      • DSCAM antibody
      • DSCAM_HUMAN antibody
      • human CHD2-52 down syndrome cell adhesion molecule 2, 9 antibody
      • OTTHUMP00000109193 2 antibody
      • SD11109p antibody
      see all

    Anti-Dscam antibody images

    • Anti-Dscam antibody (ab43847) at 1 µg/ml + Drosophila S2 Cells at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 222 kDa
      Observed band size : 235 kDa (why is the actual band size different from the predicted?)


      Exposure time : 1 minute

    References for Anti-Dscam antibody (ab43847)

    This product has been referenced in:
    • Jin XK  et al. Immunoglobulin superfamily protein Dscam exhibited molecular diversity by alternative splicing in hemocytes of crustacean, Eriocheir sinensis. Fish Shellfish Immunol 35:900-9 (2013). Read more (PubMed: 23856639) »

    See 1 Publication for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Fruit fly (Drosophila melanogaster) Tissue lysate - whole (fly heads and embryos)
    Loading amount 10 µg
    Specification fly heads and embryos
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Apr 02 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"