Overview

  • Product nameAnti-DSCC1 antibody
    See all DSCC1 primary antibodies
  • Description
    Rabbit polyclonal to DSCC1
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH conjugated synthetic peptide corresponding to a region within C terminal amino acids 318-347 of Human DSCC1 (NP_076999.2).

  • Positive control
    • CEM cell line lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab106972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.
ICC/IF 1/200. Use with paraformaldehyde-fixed cells.

Target

  • FunctionLoads PCNA onto primed templates regulating velocity, spacing and restart activity of replication forks. May couple DNA replication to sister chromatid cohesion through regulation of the acetylation of the cohesin subunit SMC3.
  • Sequence similaritiesBelongs to the DCC1 family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DCC1 antibody
    • DCC1_HUMAN antibody
    • Defective in sister chromatid cohesion 1 homolog antibody
    • Defective in sister chromatid cohesion protein 1 homolog antibody
    • DSCC1 antibody
    • hDCC1 antibody
    • MGC5528 antibody
    • Sister chromatid cohesion protein DCC1 antibody
    see all

Anti-DSCC1 antibody images

  • Anti-DSCC1 antibody (ab106972) at 1/100 dilution + CEM cell line lysate at 35 µg

    Predicted band size : 45 kDa
  • ab106972 (1/200) staining DSCC1 in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details, please refer to Abreview.

    See Abreview

References for Anti-DSCC1 antibody (ab106972)

ab106972 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 90 µg
Gel Running Conditions Reduced Denaturing (4-18% gel)
Sample Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
Specification Mouse embryonic fibroblasts
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 29 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton X100
Username

Dr. Kirk McManus

Verified customer

Submitted Sep 15 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"