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Abcam’s anti-dsDNA IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against dsDNA in Human serum and plasma.
A 96-well plate has been precoated with dsDNA antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized dsDNA-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of dsDNA IgG sample captured in plate.
|Components||1 x 96 tests|
|10X Wash Solution||1 x 50ml|
|Cover Foil||1 unit|
|dsDNA Anti-IgG HRP Conjugate||1 x 15ml|
|dsDNA Coated Microplate (12 x 8 wells)||1 unit|
|dsDNA Negative Control||1 x 1.2ml|
|dsDNA Positive Control||1 x 1.2ml|
|dsDNA Standard 0 - 0 U/mL||1 x 1.2ml|
|dsDNA Standard 1 - 12.5 U/mL||1 x 1.2ml|
|dsDNA Standard 2 - 25 U/mL||1 x 1.2ml|
|dsDNA Standard 3 - 50 U/mL||1 x 1.2ml|
|dsDNA Standard 4 - 200 U/mL||1 x 1.2ml|
|IgG Sample Diluent||1 x 100ml|
|Stop Solution||1 x 15ml|
|Strip holder||1 unit|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab178618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Indirect ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"