Overview

  • Product name
    Anti-dsDNA IgG ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Sera 32 <=3.4%
    Inter-assay
    Sample n Mean SD CV%
    Sera <=11.7%
  • Sample type
    Serum, Plasma
  • Assay type
    Indirect
  • Sensitivity
    = 0.42 U/ml
  • Range
    12.5 U/ml - 200 U/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s anti-dsDNA IgG Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of IgG class antibodies against dsDNA in Human serum and plasma.

    A 96-well plate has been precoated with dsDNA antigens to bind cognate antibodies. Controls or test samples are added to the wells and incubated. Following washing, a horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added to the wells, which binds to the immobilized dsDNA-specific antibodies. TMB is then catalyzed by the HRP to produce a blue color product that changes to yellow after adding an acidic stop solution. The density of yellow coloration is directly proportional to the amount of dsDNA IgG sample captured in plate.

  • Tested applications
    Suitable for: Indirect ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Cover Foil 1 unit
    dsDNA Anti-IgG HRP Conjugate 1 x 15ml
    dsDNA Coated Microplate (12 x 8 wells) 1 unit
    dsDNA Negative Control 1 x 1.2ml
    dsDNA Positive Control 1 x 1.2ml
    dsDNA Standard 0 - 0 U/mL 1 x 1.2ml
    dsDNA Standard 1 - 12.5 U/mL 1 x 1.2ml
    dsDNA Standard 2 - 25 U/mL 1 x 1.2ml
    dsDNA Standard 3 - 50 U/mL 1 x 1.2ml
    dsDNA Standard 4 - 200 U/mL 1 x 1.2ml
    IgG Sample Diluent 1 x 100ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Relevance
    dsDNA (double stranded deoxyribonucleic acid) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

Applications

Our Abpromise guarantee covers the use of ab178618 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Indirect ELISA Use at an assay dependent concentration.

Images

  • Typical Standard Curve
    Typical Standard Curve

    Representative standard curve using ab178618.

Protocols

References

This product has been referenced in:
  • Domljanovic I  et al. Complexes of DNA with fluorescent dyes are effective reagents for detection of autoimmune antibodies. Sci Rep 7:1925 (2017). Read more (PubMed: 28507286) »

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