Overview

  • Product nameAnti-Dsk2 antibody
  • Description
    Rabbit polyclonal to Dsk2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Saccharomyces cerevisiae
  • Immunogen

    Recombinant full length protein (S. cerevisiae). Fusion protein Dsk2 expressed in E. coli was purified and thrombin-digested full-length Dsk2 was used for antigen.

Properties

Applications

Our Abpromise guarantee covers the use of ab4119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000. Detects a band of approximately 51 kDa.

Target

  • RelevanceDsk2p is a member of a protein family that is characterized by possession of an N-terminal ubiquitin (Ub)-like (UbL) domain and a C-terminal Ub-associated (UBA) domain. In yeast, DSK2 was isolated as a suppressor of kar1, which is defective in spindle pole duplication. Although DSK2 is not an essential gene, yeast strains deleted for both DSK2 and RAD23 are temperature-sensitive for growth because of a block of spindle pole body duplication. Dsk2p and Rad23p seems to perform overlapping functions, although the sequence of Dsk2p is hardly similar to that of Rad23p overall except that they show 30% identity in their N-terminal UbL domains (residues 1-76) and that both contain UBA domains at their C terminus. Recent work on yeast Rad23p indicates that it is connected with the Ub-proteasome pathway; the N-terminal UbL domain interacts with the proteasome. There are genetic interactions between RAD23 and the polyubiquitin-binding subunit of the proteasome, RPN10, and Rad23p seems to inhibit polyubiquitin chain assembly in a cell-free assay system. It has recently been identified that Xenopus Dsk2-related protein, XDRP1, blocked Ub-dependent degradation of the cyclin A, and the human homologue of XDRP1, Plic1, is reported to form a link between the Ub machinery and the proteasome. Plic1 also is known as ubiquilin, independently isolated in a yeast two-hybrid screen by using presenilin as bait.
  • Cellular localizationNuclear
  • Database links
    • Alternative names
      • SHE4 antibody
      • Ubiquitin domain containing protein DSK2 antibody
      • YMR276W antibody

    Anti-Dsk2 antibody images

    • Lane 1: Yeast extract from dsk2 knockout strain.
      Lane 2: Yeast extract from DSK2 strain.

    References for Anti-Dsk2 antibody (ab4119)

    This product has been referenced in:
    • Funakoshi M  et al. Budding yeast Dsk2p is a polyubiquitin-binding protein that can interact with the proteasome. Proc Natl Acad Sci U S A 99:745-50 (2002). Read more (PubMed: 11805328) »

    See 1 Publication for this product

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