Overview

  • Product nameAnti-DSN1 antibody
    See all DSN1 primary antibodies
  • Description
    Mouse monoclonal to DSN1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: MTYLGSSQNE VLNTKPDYQK ILQNQSKVFD CMELVMDELQ GSVKQLQAFM DESTQCFQKV SVQLGKRSMQ QLDPSPARKL LKLQLQNPPA IHGSGSGSCQ, corresponding to amino acids 257-356 of Human DSN1 (NP_079194) with a 26 kDa tag.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab77791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionPart of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
    • Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.
    • Information by UniProt
    • Database links
    • Alternative names
      • C20orf172 antibody
      • Chromosome 20 open reading frame 172 antibody
      • dJ469A13.2 antibody
      • DSN1 antibody
      • DSN1, MIND kinetochore complex component, homolog antibody
      • DSN1, MIND kinetochore complex component, homolog (S. cerevisiae) antibody
      • DSN1, MIS12 kinetochore complex component antibody
      • DSN1_HUMAN antibody
      • FLJ13346 antibody
      • hKNL-3 antibody
      • Kinetochore associated protein DSN1 antibody
      • Kinetochore associated protein DSN1 homolog antibody
      • Kinetochore null 3 homolog antibody
      • Kinetochore-associated protein DSN1 homolog antibody
      • KNL3 antibody
      • MGC32987 antibody
      • MIS13 antibody
      see all

    Anti-DSN1 antibody images

    • Anti-DSN1 antibody (ab77791) at 1/500 dilution + recombinant fragment (the immunogen) at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/5000 dilution
      Developed using the ECL technique

      Predicted band size : 40 kDa
      Observed band size : 40 kDa

    References for Anti-DSN1 antibody (ab77791)

    ab77791 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Permeabilization Yes - 0.5% TritonX100 in PBS
    Specification HeLa
    Fixative Paraformaldehyde
    Username

    Dr. Kirk McManus

    Verified customer

    Submitted Aug 15 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"