The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: 1/500 - 1/1000. Predicted molecular weight: 40 kDa.
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionPart of the MIS12 complex which is required for normal chromosome alignment and segregation and kinetochore formation during mitosis.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Associated with the kinetochore.