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Rabbit polyclonal to DTD1
Predicted to work with:
Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human DTD1 aa 159-209. The exact sequence is proprietary. (NP_543010.3).
HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate pH 7 to 8
Concentration information loading...
Immunogen affinity purified
ab200559 was affinity purified using an epitope specific to DTD1 immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-10 µg/mg of lysate.
1/2000 - 1/10000. Predicted molecular weight: 23 kDa.
Hydrolyzes D-tyrosyl-tRNA(Tyr) into D-tyrosine and free tRNA(Tyr). Could be a defense mechanism against a harmful effect of D-tyrosine.
Expressed in many adult and fetal tissues. Highest levels in testis, ovary, spleen and in adult and fetal brain.
Belongs to the DTD family.
Information by UniProt
Western blot - Anti-DTD1 antibody (ab200559)
All lanes :
Anti-DTD1 antibody (ab200559) at 0.1 µg/ml
Lane 1 :
HeLa whole cell lysate
Lane 2 :
293T whole cell lysate
Lane 3 :
Jurkat whole cell lysate
Lane 4 :
TCMK-1 whole cell lysate
Lane 5 :
NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique
Predicted band size :
Exposure time :
Lysates prepared using NETN lysis buffer.
Immunoprecipitation - Anti-DTD1 antibody (ab200559)
Detection of DTD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab200559 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"