Overview

  • Product name
  • Description
    Rabbit polyclonal to DTD1
  • Tested applications
    Suitable for: IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Sheep, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DTD1 aa 159-209. The exact sequence is proprietary. (NP_543010.3).
    Sequence:

    QLSKLEKQQQRKEKTRAKGPSESSKERNTPRKEDRSASSGAEGDVSSERE P


    Database link: Q8TEA8

  • Positive control
    • HeLa, 293T, Jurkat, TCMK-1, and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab200559 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
WB 1/2000 - 1/10000. Predicted molecular weight: 23 kDa.

Target

Images

  • All lanes : Anti-DTD1 antibody (ab200559) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TCMK-1 whole cell lysate
    Lane 5 : NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 23 kDa


    Exposure time : 3 minutes

    Lysates prepared using NETN lysis buffer.

  • Detection of DTD1 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab200559 at 6 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

References

ab200559 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab200559.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up