Overview

  • Product nameAnti-DTHD1 antibody
  • Description
    Rabbit polyclonal to DTHD1
  • Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla
  • Immunogen

    Recombinant fragment: SLIVGDNEEL VSNVITIECS DKEKRVPFPI GIAIPFTARY RGNYRDIMVK VCDINLQSSY LNPNSLEGMK GGYKGTCASV KVYKLGIFSV VSCL, corresponding to amino acids 208-301 of Human DTHD1 (Q6ZMT9).

  • Positive control
    • WB: RT4, U251 MG, Human Plasma (weak band) and Human Liver lysates IHC-P: Human duodenum tissue

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab122412 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/10 - 1/20. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/250 - 1/500.

Target

Anti-DTHD1 antibody images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus. Recommended concentration of ab122412 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • All lanes : Anti-DTHD1 antibody (ab122412) at 1/250 dilution

    Lane 1 : RT4 cell lysate
    Lane 2 : U-251 MG cell lysate
    Lane 3 : Human Plasma lysate
    Lane 4 : Human Liver lysate
    Lane 5 : Human Tonsil lysate
    10-20% SDS PAGE
  • ab122412 at 1/10 dilution staining DTHD1 in paraffin-embedded Human duodenum tissue by Immunohistochemistry.

References for Anti-DTHD1 antibody (ab122412)

ab122412 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab122412.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"