The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.2 - 0.5 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 38 kDa). The observed much higher molecular weight corresponds to earlier findings in literature with different antibodies.
FunctionRegulator of Notch signaling, a signaling pathway involved in cell-cell communications that regulates a broad spectrum of cell-fate determinations. Probably acts both as a positive and negative regulator of Notch, depending on the developmental and cell context (By similarity). Functions as an ubiquitin ligase protein in vitro, suggesting that it may regulate the Notch pathway via some ubiquitin ligase activity.
Sequence similaritiesBelongs to the Deltex family. Contains 1 RING-type zinc finger.