Anti-Dub3 antibody (ab57801)

Overview

  • Product nameAnti-Dub3 antibody
    See all Dub3 primary antibodies
  • Description
    Mouse monoclonal to Dub3
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Recombinant Fragment
    Predicted to work with: Human
  • Immunogen

    Recombinant fragment: ESTLDHWKFL QEQNKTKPEF NVRKVEGTLP PDVLVIHQSK YKCGMKNHHP EQQSSLLNLS STTPTHQESM NTGTLASLRG RARRSKGKNK HSKRALLVCQ , corresponding to amino acids 431-531 of Human Dub3

Properties

Applications

Our Abpromise guarantee covers the use of ab57801 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notesWB: Use at a concentration of 1-5 µg/ml.

    This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionDeubiquitinating enzyme that specifically removes conjugated ubiquitin from CDC25A, thereby playing a key role in cell cycle regulation. Deubiquitination of CDC25A prevents its degradation by the proteasome during the G1/S and G2/M phases of the cell cycle and is required for cell-cycle progression. Has transforming capabilities on cell lines. May also function in cell apoptosis.
    • Tissue specificityBroadly expressed.
    • Sequence similaritiesBelongs to the peptidase C19 family. USP17 subfamily.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Cytokine inducible Deubiquitinating Enzyme antibody
      • Deubiquitinating enzyme 17-like protein 2 antibody
      • Deubiquitinating enzyme 3 antibody
      • Deubiquitinating protein 3 antibody
      • DUB-3 antibody
      • DUB3 protein antibody
      • U17L2_HUMAN antibody
      • Ubiquitin carboxyl-terminal hydrolase 17-like protein 2 antibody
      • Ubiquitin thiolesterase 17-like protein 2 antibody
      • Ubiquitin-specific-processing protease 17-like protein 2 antibody
      • USP17L2 antibody
      see all

    Anti-Dub3 antibody images

    • Western blot against tagged recombinant protein immunogen using ab57801 Dub3 antibody at 1ug/ml. Predicted band size of immunogen is 37 kDa

    References for Anti-Dub3 antibody (ab57801)

    ab57801 has not yet been referenced specifically in any publications.

    Product Wall

    DISCOUNT CODE: ***
    Expiration date: November 11, 2012
    Value: $337
    Thank you very much for your reply. I've created the code above, and it will be valid for the next month until November 11. I've also included the code in your Abreview, so...

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    Thank you very much for your email.
    Just to make sure I understand the situation, you had intended to use the code but used Abpoints instead? I will be happy to issue you a new code in this case, however we have just recently changed our testing p...

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    Thank you for your reply.
    Are you refering to 2 different antibodies: ab129931 and ab129932, or is that just a typo?
    The antibody ab129931 has been tested in WB and human samples - per our datasheet - and is guaranteed for this application an...

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    Thank you for submitting an Abreview of ab57801. As you may have noticed, your review has now been published on our website.
    Since you obtained poor results using the antibody in a tested species and application, we would like to follow up on this...

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    Abreviews
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (breast and colon cancer cell)
    Loading amount 100 µg
    Specification breast and colon cancer cell
    Gel Running Conditions Non-reduced Denaturing (10% SDS_PAGE)
    Blocking step Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 22 2012

    Thank you for contacting Abcam earlier today.
    I am sorry that you were having issues with ab57801 in western blot. As we discussed today, I am going to send ab129931 as a free of charge replacement. It is currently out of stock at the moment, but ...

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    Thank you for your call today and your interest in our Dub3 antibodies. As we discussed, I've issued the discount code below. Please let me know if you have any questions or if there is anything else that we can do for you.
    DISCOUNT CODE: ***
    ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"