A single arc was shown while the antibody was reacting with normal rabbit serum or IgG by Immunoelectrophoresis. Nothing was shown when the antibody reacted with human IgG. Similar results were observed when the antibody was tested in Western Blotting.
The affinity purified IgY (delta Fc) is purified by rabbit IgG coupling chromatography, and adsorption is performed by human serum coupling column. Using SDS PAGE (blue stain), it has been shown that the IgY(delta Fc) constitutes 90% of total protein, and the IgY and IgY(delta Fc) constitutes 95% of total protein.