Overview

  • Product name
    Anti-DULLARD antibody
  • Description
    Rabbit polyclonal to DULLARD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human DULLARD aa 138-168 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O95476

  • Positive control
    • Jurkat cell line lysates; Human stomach tissue; Jurkat cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab170765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 28 kDa.

Target

  • Relevance
    DULLARD is a serine/threonine phosphatase which may be required for proper nuclear membrane morphology. It is involved in LPIN1 dephosphorylation and may antagonize BMP signaling.
  • Cellular localization
    Endoplasmic reticulum membrane; Single-pass membrane protein. Nuclear membrane; Single-pass membrane protein
  • Database links
  • Alternative names
    • Dullard homolog antibody
    • HSA 011916 antibody
    • NET 56 antibody
    • Serine threonine protein phosphatase dullard antibody

Images

  • Flow cytometric analysis of Jurkat cells labeling DULLARD with ab170765 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Anti-DULLARD antibody (ab170765) at 1/100 dilution + Jurkat cell line lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 28 kDa

  • Immunohistochemical analysis of formalin fixed and paraffin embedded Human stomach tissue labeling DULLARD with ab170765 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab170765 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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