Overview

  • Product name
  • Description
    Goat polyclonal to DUOX1
  • Specificity
    Reported variants represent identical protein: NP_787954.1, NP_059130.2. ab178534 is not expected to cross-react with DUOX2 or DUOX3.
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human DUOX1 aa 972-985 (internal sequence). NP_059130.2.
    Sequence:

    CSRSDIETELTPQR


    Database link: Q9NRD9

  • Positive control
    • Human Testis lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab178534 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 170 kDa (predicted molecular weight: 177 kDa).

An additional band of unknown identity was also consistently observed at 140kDa. This band was successfully blocked by incubation with the immunizing peptide.

Target

  • Relevance
    The protein encoded by this gene is a glycoprotein and a member of the NADPH oxidase family. The synthesis of thyroid hormone is catalyzed by a protein complex located at the apical membrane of thyroid follicular cells. This complex contains an iodide transporter, thyroperoxidase, and a peroxide generating system that includes this encoded protein and DUOX2. This protein is known as dual oxidase because it has both a peroxidase homology domain and a gp91phox domain. Two alternatively spliced transcript variants encoding the same protein have been described for this gene (from EntrezGene).
  • Cellular localization
    Cell Membrane
  • Database links
  • Alternative names
    • Dual oxidase 1 antibody
    • Dual oxidase 1 precursor antibody
    • DUOX1 antibody
    • EC 1.11.1 antibody
    • EC 1.6.3.1 antibody
    • Flavoprotein NADPH oxidase antibody
    • Large NOX 1 antibody
    • LNOX1 antibody
    • Long NOX 1 antibody
    • MGC138840 antibody
    • MGC138841 antibody
    • NADPH thyroid oxidase 1 antibody
    • Nicotinamide adenine dinucleotide phosphate oxidase antibody
    • NOXEF1 antibody
    • THOX1 antibody
    • Thyroid oxidase 1 antibody
    see all

Images

  • Anti-DUOX1 antibody (ab178534) at 0.1 µg/ml + Human Testis lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 177 kDa
    Additional bands at : 170 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:
  • Dho SH  et al. NOX5-L can stimulate proliferation and apoptosis depending on its levels and cellular context, determining cancer cell susceptibility to cisplatin. Oncotarget 6:39235-46 (2015). Read more (PubMed: 26513170) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Western blot
Sample
Human Cell lysate - whole cell (H441)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
H441
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Jun 08 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up