Recombinant Anti-DUS2L antibody [EPR14042] (ab181262)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14042] to DUS2L
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-DUS2L antibody [EPR14042]
See all DUS2L primary antibodies -
Description
Rabbit monoclonal [EPR14042] to DUS2L -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, A549 and A431 cell lysates; HeLa cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14042 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181262 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/80.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
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IP |
1/40 - 1/60.
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ICC/IF |
1/100.
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Notes |
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Flow Cyt (Intra)
1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/2000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). |
IP
1/40 - 1/60. |
ICC/IF
1/100. |
Target
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Relevance
DUS2L is a dihydrouridine synthase that catalyzes the reduction of the 5,6-double bond of a uridine residue on the displacement loop of tRNA. The resultant modified base, 5,6-dihydrouridine, appears to increase the conformational flexibility and dynamic motion of tRNA. -
Cellular localization
Cytoplasmic and Endoplasmic reticulum. Note=Mainly at the endoplasmic reticulum. -
Database links
- Entrez Gene: 54920 Human
- Omim: 609707 Human
- SwissProt: Q9NX74 Human
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Alternative names
- 2310016K04Rik antibody
- dihydrouridine synthase 2 like, SMM1 homolog (S. cerevisiae) antibody
- DUS2 antibody
see all
Images
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All lanes : Anti-DUS2L antibody [EPR14042] (ab181262) at 1/2000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/1000 dilution
Predicted band size: 55 kDa -
Immunofluorescent analysis of HeLa cells (-20°C acetone-fixed) labeling DUS2L with ab181262 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
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Intracellular flow cytometric analysis of HeLa cells (paraformaldehyde-fixed, 2%) labeling DUS2L with ab181262 at 1/80 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.
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Western blot analysis of immunoprecipitation pellet from A549 cell lysate (lane 1) or a negative control (lane 2) immunoprecipitated using ab181262 at 1/50 dilution.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab181262 has not yet been referenced specifically in any publications.