Overview

  • Product name
  • Description
    Goat polyclonal to DUSP10
  • Specificity
    This antibody is expected to recognise isoform a (NP_009138.1) and isoform b (NP_653329.1 and NP_653330.1).
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: RILTPKLMGVETVV, corresponding to C terminal amino acids 469-482 of Human DUSP10.

Properties

Applications

Our Abpromise guarantee covers the use of ab2272 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 - 2 µg/ml. Can be blocked with Human DUSP10 peptide (ab22899).

Target

  • Function
    Involved in the inactivation of MAP kinases. Has a specificity for the MAPK11/MAPK12/MAPK13/MAPK14 subfamily.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 10 antibody
    • Dual specificity phosphatase MKP 5 antibody
    • Dual specificity phosphatase MKP5 antibody
    • Dual specificity protein phosphatase 10 antibody
    • DUS10_HUMAN antibody
    • DUSP 10 antibody
    • DUSP10 antibody
    • DUSP10 protein antibody
    • MAP kinase phosphatase 5 antibody
    • Mitogen activated protein kinase phosphatase 5 antibody
    • Mitogen-activated protein kinase phosphatase 5 antibody
    • MKP 5 antibody
    • MKP-5 antibody
    • MKP5 antibody
    • Serine/threonine specific protein phosphatase antibody
    see all

Images

  • ab2272 staining (0.5µg/ml) of Human Liver lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

References

This product has been referenced in:
  • Furman JL  et al. Blockade of Astrocytic Calcineurin/NFAT Signaling Helps to Normalize Hippocampal Synaptic Function and Plasticity in a Rat Model of Traumatic Brain Injury. J Neurosci 36:1502-15 (2016). Read more (PubMed: 26843634) »
  • Du M  et al. Transgenic Mice Overexpressing Serum Retinol-Binding Protein Develop Progressive Retinal Degeneration through a Retinoid-Independent Mechanism. Mol Cell Biol 35:2771-89 (2015). Read more (PubMed: 26055327) »

See all 3 Publications for this product

Customer reviews and Q&As

Thank you for your enquiry. This product had been tested on adult human liver lysate, made in RIPA buffer (1% TritonX100 instead of NP40). However, the product has not been tested on HepG2 or Huh-7, but I would expect similar results in HepG2. I ...

Read More

There are 3 different sequences of Dusp10 listed in the reviewed locuslink entry(http://www.ncbi.nlm.nih.gov/LocusLink/LocRpt.cgi?l=11221). Our peptide sequence is found in all three, however we never saw the smaller isoforms in WB. For that reason...

Read More

Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you dec...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up