Overview

  • Product nameAnti-DUSP16 antibody
    See all DUSP16 primary antibodies
  • Description
    Rabbit polyclonal to DUSP16
  • Specificityab65151 detects endogenous levels of total DUSP16 protein.
  • Tested applicationsSuitable for: ELISA, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide derived from an internal sequence of human DUSP16.

  • Positive control
    • Jurkat and COLO205 cell extracts and HepG2 cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab65151 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65151 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
WB 1/500 - 1/1000. Detects a band of approximately 73 kDa (predicted molecular weight: 73 kDa).

Target

  • FunctionInvolved in the inactivation of MAP kinases. Dephosphorylates MAPK10 bound to ARRB2.
  • Sequence similaritiesBelongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 rhodanese domain.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localizationCytoplasm. Nucleus. Cytoplasmic vesicle. After dissociation upon AGTR stimulation, re-associates with ARRB2 on endocytic vesicles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity phosphatase 16 antibody
    • Dual specificity protein phosphatase 16 antibody
    • DUS16_HUMAN antibody
    • DUSP 16 antibody
    • DUSP16 antibody
    • KIAA1700 antibody
    • MAP kinase phosphatase 7 antibody
    • MAPK phosphatase 7 antibody
    • Mitogen activated protein kinase phosphatase 7 antibody
    • Mitogen-activated protein kinase phosphatase 7 antibody
    • MKP 7 antibody
    • MKP-7 antibody
    • MKP7 antibody
    see all

Anti-DUSP16 antibody images

  • ab65151 at 1/500 dilution staining DUSP16 in HepG2 cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-DUSP16 antibody (ab65151) at 1/500 dilution

    Lane 1 : Jurkat cell extract
    Lane 2 : COLO205 cell extract
    Lane 3 : COLO205 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 73 kDa
    Observed band size : 73 kDa

References for Anti-DUSP16 antibody (ab65151)

ab65151 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (murine embryonic fibroblasts)
Loading amount 15 µg
Specification murine embryonic fibroblasts
Treatment 300µM hydrogen peroxide, 0.5h & 4h
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 10% · Temperature: 20°C
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Submitted May 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"