For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to DUSP22
ab70124 detects endogenous levels of total DUSP22 protein.
Synthetic peptide derived from an internal sequence of human DUSP22.
Jurkat and RAW264.7 cell extracts.
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg 2+ and Ca 2+), 150mM Sodium chloride, pH 7.4
Concentration information loading...
Immunogen affinity purified
ab70124 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml.
1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK).
Ubiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas.
Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
Contains 1 tyrosine-protein phosphatase domain.
Information by UniProt
Dual specificity protein phosphatase 22 antibody
Anti-DUSP22 antibody images
Western blot - DUSP22 antibody (ab70124)
All lanes : Anti-DUSP22 antibody (ab70124) at 1/500 dilution Lane 1 : Jurkat cell extracts Lane 2 : RAW264.7 cell extracts Lane 3 : RAW264.7 cell extracts with immunising peptide at 10 µg Lysates/proteins at 30 µg per lane. Predicted band size : 20 kDa Observed band size : 20 kDa
Immunocytochemistry/ Immunofluorescence-DUSP22 antibody(ab70124)
ICC/IF image of ab70124 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70124, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-DUSP22 antibody (ab70124)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"