Overview

  • Product name
  • Description
    Rabbit polyclonal to DUSP22
  • Specificity
    ab70124 detects endogenous levels of total DUSP22 protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from an internal sequence of human DUSP22.

  • Positive control
    • Jurkat and RAW264.7 cell extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab70124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).
ELISA 1/5000.

Target

  • Function
    Activates the Jnk signaling pathway. Dephosphorylates and deactivates p38 and stress-activated protein kinase/c-Jun N-terminal kinase (SAPK/JNK).
  • Tissue specificity
    Ubiquitous. Highest expression seen in heart, placenta, lung, liver, kidney and pancreas.
  • Sequence similarities
    Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily.
    Contains 1 tyrosine-protein phosphatase domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dual specificity protein phosphatase 22 antibody
    • DUS22_HUMAN antibody
    • Dusp22 antibody
    • JNK stimulatory phosphatase 1 antibody
    • JNK-stimulatory phosphatase-1 antibody
    • JSP 1 antibody
    • JSP-1 antibody
    • JSP1 antibody
    • LMW DSP2 antibody
    • LMW-DSP2 antibody
    • Low molecular weight dual specificity phosphatase 2 antibody
    • MAP kinase phosphatase x antibody
    • Mitogen activated protein kinase phosphatase x antibody
    • Mitogen-activated protein kinase phosphatase x antibody
    • MKP-x antibody
    • MKPX antibody
    see all

Images

  • All lanes : Anti-DUSP22 antibody (ab70124) at 1/500 dilution

    Lane 1 : Jurkat cell extracts
    Lane 2 : RAW264.7 cell extracts
    Lane 3 : RAW264.7 cell extracts with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 20 kDa
    Observed band size : 20 kDa
  • ICC/IF image of ab70124 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70124, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab70124 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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